大麻韧皮果胶分解菌株的分离与鉴定

用海水和清水对大麻纤维进行浸泡处理,9d后浸泡液用梯度稀释至体积分数为0·01%,然后涂布在培养基平板上,经培养、分离、纯化,挑选数量优势的单个菌落。分别得到1309和2508这2种具有较强果胶分解能力的菌株,这2个菌株均呈革兰氏阴性,菌株1309有数根极毛运动的直杆菌,菌株2508为单极毛运动的端圆直杆菌。通过Biolog分析,初步确定菌株1309和2508分别属于假单胞菌和寡养单胞菌属的嗜麦芽寡养单胞菌。

Separation and identification of microbes of pectin discomposing in hemp retting

The hemp bundles were submerged in clear water and sea water. After 9 days, the water used for submerging was diluted to volume fraction 0.01% by gradient method, and then applied uniformly on the culture medium sheet and the dominant single colony was selected through cultivating, separating and purifying. Thus obtained respectively two strains named 1309 and 2508 that were more effective in decomposing the pectin of the hemp. Both of the strains are Gram-negative. Strain 1309 is a bacillus with polar flagella in motion, while strain 2508 is a round-ended bacillus with polar flagellum in motion. Through biological analysis, preliminary results reveal that strain 1309 is Pseudomonas spp., and 2508 is Stenotrophomnas maltophilia.