Magnetic resonance imaging at 1.5 T with immunospecific contrast agent in vitro and in vivo in a xenotransplant model |
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Authors: | G Baio M Fabbi D de Totero S Ferrini M Cilli L E Derchi C E Neumaier |
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Affiliation: | (1) DICMI – Radiologia, University of Genova, Largo Rosanna Benzi 8, 16100 Genoa, Italy;(2) Laboratory of Immunological Therapy, IST, National Cancer Institute, Largo Rosanna Benzi 10, 16100 Genoa, Italy;(3) Animal Facility, IST, National Cancer Institute, Largo Rosanna Benzi 10, 16100 Genoa, Italy;(4) Department of Diagnostic Imaging, IST, National Cancer Institute, Largo Rosanna Benzi 10, 16100 Genoa, Italy |
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Abstract: | Object: Demonstrating the feasibility of magnetic resonance imaging (MRI) at 1.5 T of ultrasmall particle iron oxide (USPIO)-antibody
bound to tumor cells in vitro and in a murine xenotransplant model.
Methods: Human D430B cells or Raji Burkitt lymphoma cells were incubated in vitro with different amounts of commercially available
USPIO-anti-CD20 antibodies and cell pellets were stratified in a test tube. For in vivo studies, D430B cells and Raji lymphoma
cells were inoculated subcutaneously in immunodeficient mice. MRI at 1.5 T was performed with T1-weighted three-dimensional
fast field echo sequences (17/4.6/13°) and T2-weighted three-dimensional fast-field echo sequences (50/12/7°). For in vivo
studies MRI was performed before and 24 h after USPIO-anti-CD20 administration.
Results: USPIO-anti-CD20-treated D430B cells, showed a dose-dependent decrease in signal intensity (SI) on T2*-weighted images and
SI enhancement on T1-weighted images in vitro. Raji cells showed lower SI changes, in accordance to the fivefold lower expression
of CD20 on Raji with respect to D430B cells. In vivo 24 h after USPIO-anti-CD20 administration, both tumors showed an inhomogeneous
decrease of SI on T2*-weighted images and SI enhancement on T1-weighted images.
Conclusions: MRI at 1.5 T is able to detect USPIO-antibody conjugates targeting a tumor-associated antigen in vitro and in vivo. |
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Keywords: | Iron oxide particle Cell-specific MRI In vivo small animal MRI Targeted contrast material Lymphoma |
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