Optimization of the biocide properties of chitosan for its application in the design of active films of interest in the food area |
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Authors: | P Fernandez-Saiz JM Lagaron MJ Ocio |
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Affiliation: | 1. Novel Materials and Nanotechnology Laboratory, Institute of Agrochemistry and Food Technology (IATA), CSIC, Apartado de Correos, 73, 46100 Burjassot, Valencia, Spain;2. Dpto. Medicina Preventiva, Facultad de Farmacia, Universidad de Valencia, Vicent Andrés Estellés, s/n, 46100 Burjassot, Valencia, Spain |
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Abstract: | The influence on biocide performance of some unprecedented physicochemical features of chitosan cast films such as film thickness, pH of the nutrient broth, film neutralization, film autoclave sterilization and temperature exposure were analyzed against Staphylococcus aureus and in some experiments also against Salmonella spp. The work demonstrates for the first time the influence of the release or positive migration of protonated glucosamine fractions from the biopolymer into the microbial culture as the responsible event for the antimicrobial performance of the biopolymer under the studied conditions. From the results, a reliable and reproducible method for the determination of the bactericidal activity of chitosan-based films was developed in an attempt to standardize the testing conditions for the optimum design of active antimicrobial food packaging films and coating applications. |
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Keywords: | Chitosan Antimicrobial properties Biopolymers Active packaging |
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