Microscopic diagnosis of the leaf and stem of Piper solmsianum C.DC |
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Authors: | A. R. P. Bertocco I. P. Migacz V. L. P. Santos C. R. C. Franco R. Z. Silva R. A. Yunes V. Cechinel‐Filho J. M. Budel |
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Affiliation: | 1. Departamento de Ciências Farmacêuticas, Universidade Estadual de Ponta Grossa (UEPG), Ponta Grossa, Paraná, Brasil;2. Escola Superior de Saúde, Meio Ambiente, Sustentabilidade e Humanidades, Centro Universitário Internacional Uninter, Curitiba, Paraná, Brasil;3. Departamento de Biologia Celular, Universidade Federal do Paraná (UFPR), Curitiba, Paraná, Brasil;4. Departamento de Química, Universidade Federal de Santa Catarina (UFSC), Florianópolis, Santa Catarina, Brasil;5. Núcleo de Investiga??es Químico‐Farmacêuticas NIQFAR/CCS, Universidade do Vale do Itajaí (UNIVALI), Itajaí, Santa Catarina, Brasil |
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Abstract: | Piper solmsianum C.DC., which is popularly known as pariparoba, is a shrub that measures 1–3 m in height and it inhabits areas with wet tropical soils. The objective of this study was to analyze the leaf and stem anatomy using light microscopy, scanning electron micrographs, and energy‐dispersive X‐ray spectroscopy in order to provide information for species identification. The anatomical profile showed the following main microscopic markers: hypostomatic leaf; hypodermis layer on both sides; pearl glands; biconvex midrib shape; five collateral vascular bundles in open arc with the central bundle larger than the others; circular stem shape; collateral vascular bundles arranged in two rings; sinuous sclerenchymatic sheath in the pith; secretory idioblasts; and starch grains in the mesophyll, in the ground parenchyma of the midrib, petiole, and in the stem; and six morphotypes of calcium oxalate crystals (styloids, cuneiform, tabular crystal rosettes, cuneiform crystal rosettes, elongated square dipyramids, as well as very elongated square dipyramids). |
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Keywords: | anatomy energy‐dispersive X‐ray spectroscopy scanning electron microscopy |
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