离子色谱-脉冲安培测定食糖中还原糖

建立了测定食糖中还原糖(葡萄糖、果糖)的方法。以NaOH淋洗液梯度淋洗,葡萄糖、果糖在CarboPacTMPA1O高效阴离子交换柱(HPAEC)上可以在35min内完成分离。使用脉冲安培检测器(PAD)进行测定,葡萄糖、果糖的检出限(进样25μL,S/N=3)分别为1μg/L、2μg/L,且均具有较宽的线性范围(0.1～100mg/L),样品测定的相对标准偏差在0.3%～3.8%之间,加标回收率在95.8%～101.0%之间。并与滴定法所测得的还原糖含量进行了比较分析,结果偏差在13.7%以内。本方法测定还原糖分离效果好、样品前处理简单、灵敏度高,适用于食糖中还原糖的分析。

Ion Chromatography-Pulsed Amperometric Detection of Reducing sugar in Sugar

The method to determine the reducing sugar （glucose, fructose） in sugarcane was established. Eluent gradient elution with NaOH, glucose, fructose in the CarboPacTMPA10 high performance anion-exchange column （HPAEC） can be completed within 35 min in the separation. The use of pulsed amperometric detector （PAD） were measured, glucose, fructose detection limit （injection 25 pL, S/N=3） were 1 lag,q.,, 2 lag/L, and both have a wide linear range （0.1-100 mg/L）. The relative standard deviation of the sample measured at 0.3 %-3.8 %, and recoveries at between 95.8%-101.0%; and measured with the titration of the reducing sugar content of a comparative analysis of the results within a deviation of 13.7%. The determination method shows a good separation effect and high sensitivity, requires a simple sample pre-treatment, and is suitable for the analysis of reducing sugar in sugar.