负载山奈酚的小麦醇溶蛋白纳米颗粒的制备及其抗氧化活性研究

近年来,越来越多的研究证实血管病变的发生和发展与氧化应激密切相关,而山奈酚作为黄酮类化合物具有良好的抗氧化活性。本研究通过反溶剂法制备出的山奈酚-小麦醇溶蛋白纳米颗粒的粒径在200 nm左右,粒子均一,使用去离子水复溶后仍然具有良好的分散性并在28天的时间里保持其稳定性。傅里叶变换红外光谱的分析结果表明,山奈酚主要通过氢键、疏水作用以及静电作用与小麦醇溶蛋白相互结合。与游离的山奈酚相比,纳米粒子具有更好的生物相容性,在体外具有显著的抗氧化能力,可以显著地清除细胞内的活性氧。总体而言,山奈酚-小麦醇溶蛋白纳米颗粒具有良好的生物相容性,同时可以显著提高其抗氧化活性,这为山奈酚的临床应用提供了新的途径。

Preparation and Antioxidant Activity of Kaempferol-loaded Gliadin Nanoparticles

In recent years, more and more studies have confirmed that the occurrence and development of vascular diseases are closely related to oxidative stress, and kaempferol as a flavonoid has good antioxidant activity.The kaempferol- gliadin nanoparticles prepared by the anti-solvent method have a particle size of about 200 nm, uniform particles, and still have good dispersibility after reconstitution with deionized water and remain stable for 28 days . The analysis results of Fourier transform infrared spectroscopy show that kaempferol binds to gliadin mainly through hydrogen bonding, hydrophobic interaction and electrostatic interaction. Compared with free kaempferol, nanoparticles have better biocompatibility, have significant antioxidant capacity in vitro, and can significantly remove reactive oxygen species in cells.In general, kaempferol-gliadin nanoparticles have good biocompatibility and can significantly improve their antioxidant activity, which provides a new way for the clinical application of kaempferol.