Quantitative determination of butylated hydroxyanisole, butylated hydroxytoluene, and tert-butyl hydroquinone in oils, foods, and biological fluids by high-performance liquid chromatography with fluorometric detection |
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Authors: | Vivienne V Yankah Hideki Ushio Toshiaki Ohshima Chiaki Koizumi |
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Affiliation: | (1) Department of Food Science and Technology, Tokyo University of Fishories, Konan 4, Minato-ku Tokyo, 1108, Japan |
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Abstract: | Concentrations of synthetic antioxidants butylated hydroxyanisole, butylated hydroxytoluene, and tert-butyl hydroquinone were quantified using a high-performance liquid chromatograph with spectrofluorometric detector. The antioxidants
were separated and eluted on a reversed-phase column by gradient of a mixture of H2O/acetonitrile/acetic acid (66.5: 28.5∶5, by vol) and a mixture of acetonitrile/acetic acid (95∶5, vol/vol). The eluants were
monitored at emission and excitation wavelengths of 310 and 280 nm, respectively. Calibration curves obtained using peak areas
against concentration showed ligh coefficients of multiple determination (R
2>0.99) for all antioxidants. Known concentrations of added antioxidant standards were recoverable within 98–99% from oils
and over 93% from mouse blood. This method requires minimum sample extraction and purification before analysis and provides
a relatively high percentage recovery. The method has been applied successfully for the measurement of antioxidant concentrations
in oils, dried foods, and biological fluids. |
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Keywords: | |
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