| 梅花鹿茸多糖提纯 |
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| 引用本文： | 邱芳萍,都宏霞,王志兵.梅花鹿茸多糖提纯[J].哈尔滨工业大学学报,2009(7):223-225. |
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| 作者姓名： | 邱芳萍 都宏霞 王志兵 |
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| 作者单位： | 长春工业大学化学与生命科学学院 |
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| 基金项目： | 国家教育部科学技术研究重点资助项目(205036) |
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| 摘 要： | 以梅花鹿鹿茸为原料,经提取、分离、纯化得到鹿茸多糖(PAPS)粗样品,将粗品在DEAE-Cellulose 32和Sephadex G-75柱上进一步纯化,得到PAPS样品.经SDS-聚丙烯酰胺凝胶电泳检测,PAPS的相对分子量为70 kDa-80 kDa,采用苯酚-硫酸法测得总糖含量为825.51μg/mg,Folin-酚法测得蛋白质含量为20.27μg/mg,硫酸钡比浊法测得硫酸基含量为58μg/mg.红外光谱分析表明:PAPS具有明显的多糖特征吸收峰.
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| 关 键 词： | 梅花鹿 鹿茸多糖 提取 纯化 |
Purification of pilose antler polysaccharides |
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| QIU Fang-ping,DU Hong-xia,WANG Zhi-bing.Purification of pilose antler polysaccharides[J].Journal of Harbin Institute of Technology,2009(7):223-225. |
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| Authors: | QIU Fang-ping DU Hong-xia WANG Zhi-bing |
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| Affiliation: | (School of Chemical & Life Science,Changchun University of Technology,Changchun 130012,China) |
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| Abstract: | Pilose antler polysaccharides(PAPS) were obtained from pilose antler by the method of extraction,seperation and purification.The sample of PAPS was purified with the column chromatography on DEAE-Cellulose 32 and Sephadex G-75.Its molecular weight is about 70～80 kDa detected by SDS-polyacrylamide gel electrophoresis,the content of total sugar is 825.51 μg/mg determined by phenol-sulfuric acid method,the content of protein is 20.27 μg/mg determined by Foiln-hydroxybenzene method,and the content of sulfate radical is 58 μg/mg determined by barium sulfate turbidity.The sample of PAPS exhibits typical absorption of polysaccharide characterized by IR analysis. |
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| Keywords: | spotted deer pilose antler polysaccharides extraction purification |
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