| 回归正交试验法优化菜芙蓉茎中总黄酮的提取工艺 |
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| 引用本文： | 梅洪睿,云霞,杨红,徐菲,高玉峰.回归正交试验法优化菜芙蓉茎中总黄酮的提取工艺[J].大连工业大学学报,2008,27(4). |
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| 作者姓名： | 梅洪睿 云霞 杨红 徐菲 高玉峰 |
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| 作者单位： | 大连工业大学生物与食品工程学院,辽宁大连,116034;大连工业大学生物与食品工程学院,辽宁大连,116034;大连工业大学生物与食品工程学院,辽宁大连,116034;大连工业大学生物与食品工程学院,辽宁大连,116034;大连工业大学生物与食品工程学院,辽宁大连,116034 |
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| 摘 要： | 研究了菜芙蓉茎中黄酮类物质的分离提取方法,用回归正交试验法优化了提取条件,采用吸光值法、薄层层析法和高效液相色谱法定性定量分析了提取液。优化的提取条件为:乙醇体积分数69%,提取温度61.5℃,料液比为1∶41.5(mg/mL),提取时间4.8 h。此条件下黄酮类物质的提取率为1.023 mg/g。
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| 关 键 词： | 菜芙蓉 黄酮 回归正交试验 |
Optimization on extraction methods of flavonoids in stalk of Hibiscus manihot with orthogonal regression |
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| MEI Hong-rui,YUN Xia,YANG Hong,XU Fei,GAO Yu-feng.Optimization on extraction methods of flavonoids in stalk of Hibiscus manihot with orthogonal regression[J].Journal of Dalian Dalian Polytechnic University,2008,27(4). |
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| Authors: | MEI Hong-rui YUN Xia YANG Hong XU Fei GAO Yu-feng |
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| Abstract: | The extracting condition on flavonoids from Hibiscus manihot was optimized by orthogonal regression experimental design methods.The extract was quantitatively and qualitatively analyse by absorption,TLC method and HPLC.The optimum extracting conditions were 69% alcohol as solvent,extracting temperature 61.5 ℃,stalk material to solvent ratio 1∶41.5,extracting time 4.8 h.The maximal extracting rate of flavonoids was 1.023 mg/g of dry weight. |
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| Keywords: | Hibiscus manihot flavoniods orthogonal regression methods |
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