季德胜蛇药片对大鼠肺缺血再灌注损伤的保护作用

目的: 探讨季德胜蛇药片对大鼠肺缺血再灌注损伤的保护作用及其机制。方法: 将30只清洁级SD雄性大鼠分为季德胜蛇药组(D组)、对照组(C组)和缺血再灌注组(I/R组),每组10只。建立大鼠肺缺血再灌注模型,检测大鼠肺组织湿干重比;光学显微镜及透射电镜下观察肺组织损伤程度;通过明胶酶谱法测定肺组织匀浆液中明胶酶即金属蛋白酶-2、-9(MMP-2、MMP-9)的活力;逆转录酶PCR方法检测MMP-2、MMP-9 mRNA在大鼠肺组织中的表达变化。结果: 与缺血再灌注组比较,蛇药组肺湿干重比降低(P<0.01),肺组织损伤程度减轻,肺组织匀浆液MMP-9酶活力显著下降,MMP-2酶活力略有降低,肺组织中MMP-9、MMP-2的mRNA表达均降低。 结论: 季德胜蛇药片通过抑制MMP-9、MMP-2的活性及表达,使基底膜的降解降低,从而对大鼠肺缺血再灌注损伤有一定的保护作用。

The protective effects of Jidesheng Sheyao pian on lung ischemia-reperfusion injury in rats

AIM: To investigate the protective effects and the mechanisms of Jidesheng Sheyao pian on lung ischemia-reperfusion injury of rats.METHODS: Thirty SD male rats were divided into Jidesheng Sheyao group (n=10), control group (n=10) and ischemia-reperfusion injury group (I/R, n=10). The models of lung ischemia-reperfusion injury of SD rats were established. Then, the wet/dry ratio was measured, the histopathology of the lung injury was observed by light microscope and electron microscope, the activity of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9 (MMP-9) in the lung homogenates were detected by zymography, RT-PCR the expression of MMP-2, MMP-9 mRNA were detected in rat lung tissues.RESULTS: Compared with the I/R group, the wet/dry ratio were significantly decreased (P<0.01), the degree of lung injury showed lighter, the activity of MMP-9 in lung homogenate presented inhibition (P<0.01) in the drug group and the expression of MMP-9, MMP-2 mRNA was also depressed than that of the I/R group.CONCLUSION: Jidesheng Sheyao pian can inhibit the expression and activity of MMP-9 and MMP-2, attenuate basement membrane degradation, sequentially, has protective effects on lung ischemia-reperfusion injury in rats.