紫外诱变和丁醇驯化复合选育高产丁醇菌株

本研究以Clostridium beijerinckii NCIMB 8052为出发菌株,利用紫外诱变和高丁醇环境驯化相结合的方法复合选育,最终获得一株耐丁醇的高产突变株,命名为Clostridium beijerinckii ZL01.与出发菌株8052相比,ZL01对丁醇初始浓度的耐受能力从10g/L提高到11g/L.5L发酵罐的分批发酵结果表明,丁醇的产量从10.34g/L增加为15.01g/L,提高45.16％;总溶剂从12.87g/L增加为19.55g/L,提高49.01％;发酵周期缩短4h,发酵强度从0.27g/(L·h)提高为0.44g/(L·h),遗传稳定性实验表明,该菌株连续传代20次,溶剂产量稳定,菌株无明显退化.

Screening a butanol-high production strain by UV mutation and butanol domestication

Clostridium beijerinckii NCIMB 8052 was taken as the original strain in this study, treated with UV mutation and butanol domestication. Finally we have got a mutant which has high butanol tolerance and butanol production, named Clostridium beijerinckii ZL01. The butanol tolerance for this mutant ZL01 was increased to 11g/L, and which was 10g/L for the original strain. The results for 5L batch fermentation showed that ZL01 produced 15.01g/L butanol and 19.55g/L total solvents, respectively, which were 45.16% and 49.01% higher than the original strain. The fermentation period was shortened by 4h than the wild-type strain. The productivity increased from 0.27g/(L·h) to 0.44g/(L·h). The Genetic stability experiment showed that ZL01 could be serially passaged 20 times and maintained stable solvents production.