盐酸氨基乙酰丙酸搽剂在中重度痤疮患者中的局部聚集作用研究

目的:研究盐酸氨基乙酰丙酸搽剂在中重度痤疮患者面部患处的局部聚集情况,从而确定最佳搽剂浓度、给药后最佳起始光照时间和严格避光时间。方法:分为2个阶段进行研究,第1阶段24例中重度痤疮患者随机分为3组,分别给予3种浓度搽剂后照光,采集用药部位荧光图像进行分析,确定最佳搽剂浓度、给药后最佳起始光照时间。第2阶段8例中重度痤疮患者,按最佳搽剂浓度和最佳起始光照时间给药和照光,采集用药部位荧光图像进行分析,同时采集血浆及尿液并测定其中氨基乙酰丙酸(ALA)和原卟啉Ⅸ的浓度,确定给药后严格避光时间。结果:第1阶段结果显示药物在患处有较强的聚集选择性,3个浓度组荧光强度在4~8 h 达到或接近最大值,10%浓度组荧光强度在4~8 h 高于其他浓度组。第2阶段结果显示血浆和尿液中均未检测到ALA及原卟啉Ⅸ,药物洗净后 24 h 受试者患处平均荧光强度约为洗净后0 h平均荧光强度的43%,提示药物洗净后需严格避光超过 24 h。结论:最佳搽剂浓度为10%,最佳起始光照时间为4~8 h,从减少患者治疗时间考虑起始光照时间可选为 4 h,严格避光时间持续至药物洗净后 48 h。

Topical accumulation of aminolevulinic acid in moderate to severe acne patients

AIM: To study topical accumulation of aminolevulinic acid in affected parts of moderate to severe acne patients and to determine the optimal liniment concentration,optimal time of beginning lighting and duration time of protecting from light.METHODS: The study was divided into two parts.24 moderate to severe acne patients were divided into three groups in the first part.High,middle and low concentration liniment was given to three groups respectively.Optimal liniment concentration and optimal time of beginning lighting were determined by collecting the facial fluorescence images.8 moderate to severe acne patients were enrolled in the second part. Optimal liniment concentration and optimal initial light time were applied in this part.Plasma and urine samples were also collected to detect concentration of ALA and protoporphyrin Ⅸ.Duration time of protecting from light was determined by collecting the facial fluorescence images.RESULTS: Aminolevulinic acid was absorbed and aggregated in affected parts.Fluorescence intensity reached to approximate maximum during 4-8 h for three groups. Fluorescence intensity of the 10% group is larger than other groups during 4-8 h in the first part.ALA and protoporphyrin Ⅸ were not detected in plasma and urine.Fluorescence intensity in 24 h after clearing the drug approximate 43% comparing with 0 h. Duration time of protecting from light was more than 24 h after clearing the drug.CONCLUSION: Optimal liniment concentration of aminolevulinic acid was 10%.Optimal time of beginning lighting was during 48 h. The optimal time of beginning lighting is 4 h.Duration time of protecting from light was 48 h after clearing the drug.