| 二次正交旋转组合设计优化青霉产α-葡萄糖苷酶培养基 |
| |
| 引用本文： | 陈桂光,符佳精,谷岭丽,梁钰婷,梁智群. 二次正交旋转组合设计优化青霉产α-葡萄糖苷酶培养基[J]. 中国酿造, 2013, 32(6): 105 |
| |
| 作者姓名： | 陈桂光 符佳精 谷岭丽 梁钰婷 梁智群 |
| |
| 作者单位： | 广西大学生命科学与技术学院,广西南宁,530000 |
| |
| 摘 要： | 优化青霉产α-葡萄糖苷酶培养基的3个因素配比.应用DPS v7.05统计分析软件中的三元二次正交旋转组合设计方案进行试验设计.通过二次多项回归方程寻优,当麦芽糖5.5％、蛋白胨1.25％、麸皮汁:土豆汁3∶2,此时的酶活力达到最大4.23u/ml,比优化前酶活力2.49u/ml提高了41.1％.通过产酶验证试验得出实验值与模型预测值基本符合,说明二次正交旋转组合设计可用于产酶培养基组分的优化和分析.
|
| 关 键 词： | 二次正交旋转组合设计 α-葡萄糖苷酶 培养基 青霉 |
Optimization production α-glucosidase medium of Penicillium by quadratic orthogonal rotation combination design |
| |
| CHEN Guiguang,FU Jiajing,GU Lingli,LIANG Zhiqun. Optimization production α-glucosidase medium of Penicillium by quadratic orthogonal rotation combination design[J]. China Brewing, 2013, 32(6): 105 |
| |
| Authors: | CHEN Guiguang FU Jiajing GU Lingli LIANG Zhiqun |
| |
| Affiliation: | College of Life Science and Technology, Guangxi University, Nanning 530000, China |
| |
| Abstract: | Optimized the formula of three ingredients of medium for Penicillium production of α-glucosidase, methods design the test by using the quadratic orthogonal rotation combination design in DPS software. Results the optimization of quadratic regression equation was in maltose 5.5%, peptone 1.25%, bran juice∶potato juice 3∶2, enzyme activity reach maximum at this time, increased 41.1% the enzyme activity than before. Conclusion quadratic orthogonal rotation combination design was suitable for the optimization of ingredients of fermentation medium. |
| |
| Keywords: | quadratic orthogonal rotation combination design α-glucosidase medium Penicillium |
|
| 点击此处可从《中国酿造》浏览原始摘要信息 |
|
点击此处可从《中国酿造》下载全文 |
|
