不同品系小鼠对mCPP诱导焦虑样行为的差异研究

目的: 评价BALB/c和ICR小鼠对1-(3-氯苯基)哌嗪单盐酸盐(mCPP)诱导焦虑样行为的差异。方法: 将两品系小鼠各分为四组:生理盐水对照组、mCPP焦虑组、地西泮对照组和地西泮+mCPP组。行为学检测前30 min腹腔注射地西泮、皮下注射mCPP,采用开场实验、明暗穿箱和高架十字迷宫比较两品系小鼠焦虑样行为的差异。结果: 地西泮使焦虑的BALB/c小鼠中央路程比值和中央时间比值增加,对ICR小鼠无作用;两品系小鼠运动总路程有差异。地西泮使焦虑小鼠进入暗箱潜伏期延长、明暗穿箱次数增加、暗箱滞留时间缩短,BALB/c小鼠对地西泮的反应比ICR小鼠更敏感。地西泮可使焦虑小鼠开臂进入次数百分比、开臂停留时间百分比和开臂探臂时间百分比增加,两品系小鼠仅在开臂停留时间和开臂探臂时间上有差异。结论: mCPP诱导焦虑小鼠比正常小鼠对地西泮的敏感性高;BALB/c小鼠焦虑水平较低,探究能力和风险评估能力较高,是抗焦虑药物研究中可供选择的较合适的动物品系。

Differences in anxiety-related behavior induced by mCPP and responses to diazepam in two strains of mice

AIM: To compare the differences in anxiety-related behavior induced by chlorophenylpiperazinehydrochloride (mCPP) and response to diazepam in BALB/c mice and ICR mice.METHODS: Each were divided into four groups: saline group, mCPP group, diazepam group and (diazepam+mCPP) group. Diazepam was administrated intraperitoneally and mCPP was administrated subcutaeously 30 min before behavioral test. Open-field test, light-dark transition test and elevated plus maze test were used.RESULTS: In open-field test, the central distance ratio and the central time ratio were increased in the (diazepam+mCPP) group of BALB/c mice. But there were no effects on ICR mice.Only was there significant difference on total distance between two mice strains.In light-dark transition test, latency into dark box and number of transitions were increased, and time ratio in dark box was decreased in (diazepam+mCPP) group of two strains mice. The response to diazepam was were more sensitive in BALB/c mice than ICR mice. Number of open-arm entries, time and exploring time ratio in open-arm in the elevated plus maze all increased in (diazepam+mCPP) group of two strains mice. There were differences of time and exploring time in open-arm between them.CONCLUSION: The response to diazepam is more sensitive in anxiety mice than normal mice.BALB/c mice have low level of anxiety, high baseline exploration and risk assessment of a new environment, which is a suitable mice strain to develop anxiolytic drugs.