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| 一株产β-葡萄糖苷酶菌株的筛选及酶学性质研究 | |
| 引用本文： | 刘德海,郝益民,岳丹丹,马焕,权淑静,丁冉冉,陈国参.一株产β-葡萄糖苷酶菌株的筛选及酶学性质研究[J].中国酿造,2013,32(6):47. |
| 作者姓名： | 刘德海 郝益民 岳丹丹 马焕 权淑静 丁冉冉 陈国参 |
| 作者单位： | 河南省科学院生物研究所有限责任公司,河南郑州450008;河南省工业酶工程技术研究中心,河南郑州450008;河南省环境保护科学研究院,河南郑州,450004;河南省科学院生物研究所有限责任公司,河南郑州,450008;河南农业大学,河南郑州,450002 |
| 摘 要： | 研究以降解纤维二糖菌株为主要筛选对象,从酿造大曲样品中分离筛选获得产β-葡萄糖苷酶的真菌菌株D8,并对其进行了生物学鉴定,将其鉴定为梨头霉属(Absidia)的伞枝梨头霉菌(Lichtheimia corymbifera),并对其发酵所产β-葡萄糖苷酶酶学性质进行了研究,为其开发研究提供参考. |
| 关 键 词： | β-葡萄糖苷酶 伞枝梨头霉 18S rDNA 筛选 酶学性质 |
Isolation of a strain with β-glucosidase and its enzymatic properties |
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| LIU Dehai,HAO Yimin,YUE Dandan,MA Huan,QUAN Shujing,DING Ranran,CHEN Guocan.Isolation of a strain with β-glucosidase and its enzymatic properties[J].China Brewing,2013,32(6):47. | |
| Authors: | LIU Dehai HAO Yimin YUE Dandan MA Huan QUAN Shujing DING Ranran CHEN Guocan |
| Affiliation: | 1.Henan Academy of Science Institute of Biology Co., Ltd., Zhengzhou 450008, China; 2.Henan Engineering Research Center of Industrial Enzymes, Zhengzhou 450008, China; 3.Henan Research Academy of Environmental Sciences, Zhengzhou 450004, China; 4.College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China |
| Abstract: | A fungi strain D8 with high yield of β-glucosidase was isolated from brewing koji in the laboratory using the cellobiose as the only carbon source in fermentation medium. By the characterization of morphological and the analysis of 18S rDNA sequences, D8 was identified as Lichtheimia corymbifera. The enzymatic propreties of L. corymbifera D8 were studied to provide some reference for its development and research. |
| Keywords: | β3-glucosidase Lichtheimia corymbifera 18S rDNA isolation enzymatic property |
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