胶原蛋白酶产生菌的筛选及初步鉴定 |
| |
引用本文: | 邓加聪,郑虹,陈丽琴. 胶原蛋白酶产生菌的筛选及初步鉴定[J]. 中国酿造, 2013, 32(4): 78 |
| |
作者姓名: | 邓加聪 郑虹 陈丽琴 |
| |
作者单位: | 福建师范大学福清分校生物与化学工程系,福建福清,350300 |
| |
摘 要: | 从养殖场附近淤泥中采样,分离得到20株胶原蛋白水解活性的菌株.经活性平板初筛、摇床复筛及酶活测定,筛选得到1株胶原蛋白酶活性为28.70U/mL的菌株.通过对该菌株16S rDNA的blast检索及序列分析表明,该菌株与蜡状芽孢杆菌属有99%以上的同源性,结合生理生化实验结果,经形态观察、生化试验,初步鉴定该菌株为蜡状芽孢杆菌.
|
关 键 词: | 胶原蛋白 胶原蛋白酶 菌株筛选 |
Screening and preliminary identification of collagenase-producing bacteria |
| |
Abstract: | Twenty strains with collagen hydrolyzation activity were isolated from the soil of the pig mark. The strain with collagenase activity about 28.70U/ml was obtained by primary plate screening, secondary incubator screening and enzyme activity assay. Blast analysis showed that the 16S rDNA of the strain was 99% similarity with Bacillus cereus . The result including biochemical tests indicated that the stain MAP-K5 was belonged to Bacillus cereus. |
| |
Keywords: | collagen collagenase strain screening |
|
| 点击此处可从《中国酿造》浏览原始摘要信息 |
|
点击此处可从《中国酿造》下载全文 |
|