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葛根素对庆大霉素致大鼠肾毒性的保护作用
引用本文:金婉冰. 葛根素对庆大霉素致大鼠肾毒性的保护作用[J]. 金属学报, 2013, 18(8): 864-867
作者姓名:金婉冰
作者单位:浙江省食品药品检验研究院,杭州 310004,浙江
摘    要:目的: 研究葛根素(Puerarin, Pue)对庆大霉素大鼠肾毒性的保护作用。方法: 雄性SD大鼠给予溶媒或葛根素(50 mg·kg-1·d-1)7 d 后,再联合给予 0.9%氯化钠注射液或庆大霉素 140 mg/kg (i.p.)14 d,测定大鼠体重,血清尿素氮(BUN)、血肌酐(SCr)含量,计算肌酐清除率(CCr),测定肾组织中丙二醛(MDA)和一氧化氮(NO)含量和超氧化物歧化酶(SOD)及内皮一氧化氮合酶(eNOS)活性;HE染色观察肾脏病理改变。结果: 与正常对照组比较,庆大霉素组可致大鼠体重、CCr、NO和 SOD活性下降,血清BUN和SCr及肾组织MDA含量升高,肾脏病理改变明显。给予葛根素明显减轻上述改变。结论: 葛根素有效拮抗庆大霉素所致的大鼠肾损害,可能通过其抗氧化作用及提高NO水平来发挥作用。

关 键 词:葛根素  庆大霉素  肾毒性  超氧化物歧化酶  丙二醛  一氧化氮  
收稿时间:2013-03-16
修稿时间:2013-06-21

Protective effects of puerarin on gentamicin-induced nephrotoxicity in rats
JIN Wan-bing. Protective effects of puerarin on gentamicin-induced nephrotoxicity in rats[J]. Acta Metallurgica Sinica, 2013, 18(8): 864-867
Authors:JIN Wan-bing
Affiliation:Zhejiang Research Institute for Food and Drug Control, Hangzhou 31000, Zhejiang, China
Abstract:AIM: To explore the possible protective effects of puerarin (Pue) against gentamicin (GM)-induced nephrotoxicity in rats.METHODS: Male rats were i.p. administrated of Pue (50 mg·kg-1·d-1) or vehicle (0.9% NaCl) for 7 days before and 14 days concurrently with saline or GM (140 mg /kg, i.p.). Body weight, serum levels of creatinine (SCr) and blood ureanitrogen (BUN) and creatinine clearance rate (CCr) were assessed, the level of malondialdehyde (MDA), nitric oxide (NO), the activities of superoxide dismutase (SOD) and endothelial nitric oxide synthase (eNOS) in renal tissue were determined. The renal histopathological changes were observed by hematoxylin-eosin staining.RESULTS: Compared with the control group, rats treated with GM showed decrease in body weight, Ccr, NO and the SOD activity in kidney, and a significant elevation of serum SCr, BUN and the renal MDA contents and severe morphology changes. Pue significantly reversed the changes mentioned above.CONCLUSION: Pue provide marked protective effect against GM-induced nephrotoxicity in rats, which may be related to their antagonistic effects on oxidative stress, and the increment of NO level.
Keywords:Puerarin  Gentamicin  Nephrotoxicity superoxide dismutase  Malond ialdehyde  Nitric oxide  
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