| 高效液相色谱法测定饮料中的4-甲基咪唑 |
| |
| 引用本文： | 张晓燕,芦春梅,郭娜,胡婷婷. 高效液相色谱法测定饮料中的4-甲基咪唑[J]. 中国酿造, 2013, 32(5): 148 |
| |
| 作者姓名： | 张晓燕 芦春梅 郭娜 胡婷婷 |
| |
| 作者单位： | 吉林大学药学院,吉林长春,130062;吉林省出入境检验检疫局,吉林长春,130062;吉林大学,吉林长春,130062 |
| |
| 摘 要： | 建立了高效液相色谱法检测饮料中的4-甲基咪唑的检测方法.样品经过萃取、洗涤,利用高效液相色谱仪在波长210nm处检测.该方法对4-甲基咪唑的检测限为1.0mg/kg,在1μg/mL～ 100μg/mL的范围内有很好的线性,样品的加标回收率为(80.90±0.05)％～(115.50±0.05)％.该方法有很好的准确度和重复性,为各种饮料中4-甲基咪唑的检测提供了依据.
|
| 关 键 词： | 高效液相色谱法 4-甲基咪唑 饮料 |
Determination of 4-methylimidazole in drink by high performance liquid chromatography |
| |
| ZHANG Xiaoyan,LU Chunmei,GUO Na,HU Tingting. Determination of 4-methylimidazole in drink by high performance liquid chromatography[J]. China Brewing, 2013, 32(5): 148 |
| |
| Authors: | ZHANG Xiaoyan LU Chunmei GUO Na HU Tingting |
| |
| Affiliation: | 1.College of Pharmaceutical Sciences, Jilin University, Changchun130000, China; 2.Jilin Entry-Exit Inspection & Quarantine Bureau, Changchun 130000, China; 3.Jilin University, Changchun 130000, China |
| |
| Abstract: | A sensitive method was developed for the determination of 4-methylimidazole in drink by high performance liquid chromatography（HPLC）. The samples were processed by extraction and washing, and detected at 210nm by HPLC. Under the optimized conditions, the method detection limit was 1.0mg/kg and the linear range was 1μg/ml~100μg/ml. The recoveries of 4-methylimidazole at three spiked concentration levels ranged from (80.90±0.05)% to (115.50±0.05)%. The results show that the method is accurate for analysis of 4-methylimidazole in drink and provide the basis for determination of 4-methylimidazole. |
| |
| Keywords: | high performance liquid chromatography 4-methylimidazole drink |
|
| 点击此处可从《中国酿造》浏览原始摘要信息 |
|
点击此处可从《中国酿造》下载全文 |
|
