| 部分茶饮料原辅料中优势菌-克罗诺杆菌(原阪崎肠杆菌)的分离和鉴定 |
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| 引用本文： | 陈雅蘅,赵炜,王洋,周帼萍.部分茶饮料原辅料中优势菌-克罗诺杆菌(原阪崎肠杆菌)的分离和鉴定[J].中国酿造,2013,32(5):59. |
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| 作者姓名： | 陈雅蘅 赵炜 王洋 周帼萍 |
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| 作者单位： | 武汉工业学院生物与制药工程学院,湖北武汉,430023;南通出入境检验检疫局,江苏南通,226002 |
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| 摘 要： | 克罗诺杆菌(原阪崎肠杆菌)是新兴的条件致病菌,主要通过污染的婴儿配方奶粉引起2岁以下婴幼儿严重疾病甚至死亡.在对茶饮料原辅料192份样品进行细菌总数测定和优势菌16S rDNA序列分析时,发现2批次绿茶和l批次果葡糖浆中优势菌疑似条件致病菌-克罗诺杆菌,而且其数量较高(4.0× 102cfu/g～104cfu/g).进而通过rpoB序列分析,将4个分离株鉴定为阪崎克罗诺杆菌Cronobacter sakazakii(原阪崎肠杆菌Enterobacter sakazakii).结果表明在常见的食品原料-果葡糖浆和绿茶中存在克罗诺杆菌的污染,rpoB分型方法在对克罗诺杆菌属细菌的鉴定到种上表现出高度特异性,灵敏而准确.
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| 关 键 词： | 克罗诺杆菌 阪崎肠杆菌 茶饮料原辅料 16S rDNA序列分析 rpoB序列分析 |
Isolation and identification of predominant bacteria-Cronobacter sakazakii from some tea drink materials |
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| CHEN Yaheng,ZHAO Wei,WANG Yang,ZHOU Guoping.Isolation and identification of predominant bacteria-Cronobacter sakazakii from some tea drink materials[J].China Brewing,2013,32(5):59. |
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| Authors: | CHEN Yaheng ZHAO Wei WANG Yang ZHOU Guoping |
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| Affiliation: | 1.Institute of Bioengineering and Pharmaceutical Engineering, Wuhan Polytechnic University,Wuhan 430023, China;
2.Nantong Entry-Exit Inspection and Quarantine Bureau, Nantong 226002, China |
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| Abstract: | Cronobacter is an emerging opportunistic bacterial pathogen which could cause serious neonatal diseases by contaminated powdered infant formula. During the investigation of microbiology in tea-drink materials from 2010-2011, 192 samples were analyzed by routine colony counts and 16 S rDNA sequence analysis of predominant bacteria. Three samples were found predominated by 4.0×102->104cfu/g Cronobacter like bacteria. And it was confirmed by 16S rDNA and rpoB sequence analysis that those four isolates were C. sakazakii (formerly E. sakazakii ). It were showed that normal food materials- green tea and fructose syrup could also contaminated by Cronobacter, and rpoB sequence analysis is capable to differential several species within this genus. |
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| Keywords: | Cronobacter sakazakii Enterobacter sakazakii tea drink materials 16S rDNA sequence analysis rpoB sequence analysis |
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