一株高产油脂酵母菌株的分离鉴定及其油脂组分的分析

从造纸厂工业废水中筛选到高产油脂酵母菌株H810,该菌株在以葡萄糖为唯一碳源的限氮基础培养基中进行发酵时,菌体内油脂含量和产量最高分别达到42.80％和6.54g/L.通过经典形态学、生理生化特征和26S rDNA D1/D2序列系统进化树分析,初步鉴定菌株H810为粘红酵母(Rhodotorula glutinis).气相色谱分析了菌株H810所产油脂的脂肪酸组分,其中含有大量多不饱和脂肪酸,油酸(40.23％)、棕榈酸(15.76％)、亚油酸(14.86％)、a-亚麻酸(12.23％)和γ-亚麻酸(7.23％),所产的多不饱和脂肪酸在医药保健领域具有广泛的应用.

Isolation and identification of a high-lipid yeast and its lipid component analysis

A high lipid yeast H810 was isolated from industrial wastewater papermaking factory.Using glucose as sole carbon souse tnder nitrogen-limiting culture conditions,intracellular lipid content and the highest lipid yield reached 42.80％ and 6.54g/L,respectively.This strain H810 was identified preliminarily as Rhodotorula glutinis through the analysis of classical morphology,physi-biochemical approaches and 26S rDNA D1/D2 sequence of phylogenetic tree.Gas chromatography analysis demonstrated that the fatty acid composition of lipid by strain H810 contains a lot of polyunsaturated fatty acid,such as oleic acid (40.23％),palmitic acid (15.76％),linoleic acid (14.86％),α-linolenic acid (12.23 ％) and γ-linolenic acid (7.23％).The polyunsaturated fatty acid have been widely used in the medicine and health field.