| 产蛋白酶海洋微生物的筛选鉴定及发酵 |
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| 引用本文： | 崔少飞,董超,史延茂,刘丹,崔小颖. 产蛋白酶海洋微生物的筛选鉴定及发酵[J]. 中国酿造, 2013, 32(6): 110 |
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| 作者姓名： | 崔少飞 董超 史延茂 刘丹 崔小颖 |
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| 作者单位： | 河北工业大学化工学院,天津300130;河北省科学院生物研究所,河北石家庄050081;河北省科学院生物研究所,河北石家庄,050081;河北工业大学化工学院,天津,300130 |
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| 摘 要： | 从秦皇岛附近海域水样中筛选出了1株蛋白酶生产菌,并对其发酵条件进行了初步研究.经16S rDNA鉴定此菌株属假交替单胞菌属(Pseudoalteromonas sp.),所产蛋白酶为中性蛋白酶.液体培养基为人工海水,蛋白胨10.0g/L,酵母浸粉5.0g/L,葡萄糖5.0g/L,硝酸铵1.0g/L,无水氯化钙1.0g/L,在33℃,初始pH值6.0,50mL装液量,200r/min条件下发酵,发酵液酶活可达1140 U/mL.
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| 关 键 词： | 海洋微生物 蛋白酶 菌种筛选 |
Screening and identification of protease-producing marine microorganism and its fermentation conditions for alkaline protease production |
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| CUI Shaofei,DONG Chao,SHI Yanmao,LIU Dan,CUI Xiaoying. Screening and identification of protease-producing marine microorganism and its fermentation conditions for alkaline protease production[J]. China Brewing, 2013, 32(6): 110 |
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| Authors: | CUI Shaofei DONG Chao SHI Yanmao LIU Dan CUI Xiaoying |
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| Affiliation: | 1. College of Chemical Industry, Hebei University of Industry, Tianjin 300130, China;
2. Institute of Biological Science, Hebei Academia of Science, Shijiazhuang 050081, China |
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| Abstract: | A strain of protease-producing marine microorganism was isolated from the sea-water in Qinhuangdao. The marine microorganism was identified belonging to the genus of Pseudoalteromonas sp., and the protease which produced by the strain was neutral. The optimum broth was consisted of peptone 10.0g/L, yeast extract powder 5.0g/L, glucose 5.0g/L, NH4NO3 1.0g/L, CaCl2 1.0g/L. Enzyme activity could reach 1140U/ml under the fermentation condition: fermentation temperature 33℃, initial pH 6.0, filling volume 50 ml, rotational speed 200 r/min. |
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| Keywords: | marine microorganisms protease screening |
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