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| 引用本文： | 李海静,吴胜明,方均建,李昱,马昆鹏,宋占军,董方霆.???????÷?????????????????[J].质谱学报,2009,30(2):83-87. |
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| 作者姓名： | 李海静 吴胜明 方均建 李昱 马昆鹏 宋占军 董方霆 |
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| 作者单位： | ??????????????????????? 100850 |
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| 摘 要： | ???????????-????????????(GC-TOF-MS)????????????????(FFA)?????о?????????????????????????????????-????/????????????/???????????????м????????????????????????GC-TOF-MS???????????????δ????????????????????о????????????????????????33min??????????????/?????????????????20??C12??C24????????????????????????FFA?????????÷????????????????????????о??????μ??????????Ч?????????????????????????????????
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| 关 键 词： | ?????????(FFAs) ????? ???????-???????????(GC-TOF-MS) ?????? |
Determination of Free Fatty Acids in Human Serum by GC-TOF-MS |
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| LI Hai-jing,WU Sheng-ming,FANG Jun-jian,LI Yu,MA Kun-peng,SONG Zhan-jun,DONG Fang-ting.Determination of Free Fatty Acids in Human Serum by GC-TOF-MS[J].Journal of Chinese Mass Spectrometry Society,2009,30(2):83-87. |
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| Authors: | LI Hai-jing WU Sheng-ming FANG Jun-jian LI Yu MA Kun-peng SONG Zhan-jun DONG Fang-ting |
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| Affiliation: | National Center of Biomedical Analysis, Beijing 100850, China |
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| Abstract: | Amodifiedmethodfordeterminationoffreefattyacids(FFAs)inhumanserumwasestablishedbygaschromatographytime-offlightmassspectrometry(GC-TOF-MS).Heptadecanoicacidmethylesterwasselectedasinternalstandard.Afterderivatizationby2.5%H2SO4/CH3OH,theFFAsinserumweretransformedintothefattyacidmethylesters,and N-hexanewasaddedtoextracttheproducts.TheanalysisofextractwasperformedbyGC-TOF-MS.Betterresultswereobtainedwithderivatizationcomparingwithnon-derivatization.Atleast20kindsofFFAs(C12-C24)inhumanserumaredeterminatedbythemodifiedmethod.Theanalysisiscompletedwithin33min.ThemodifiedmethodshowspotentialusefulforthescientificresearchtothediseasecorrelatingFFAsandlipidomicsduetothesimplify,rapidity,highaccuracyandenormousinformation. |
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| Keywords: | freefattyacids(FFAs) humanserum gaschromatographytime-offlightmassspectrometry(GC-TOF-MS) derivatization |
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