| BCG-CpG-DNA免疫活性作用的初步研究 |
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| 引用本文： | 赵爱华,寇丽杰,乔来艳,贾淑珍,王国治.BCG-CpG-DNA免疫活性作用的初步研究[J].粉末涂料与涂装,2006,19(6):574-577. |
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| 作者姓名： | 赵爱华 寇丽杰 乔来艳 贾淑珍 王国治 |
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| 作者单位： | 中国药品生物制品检定所菌苗室 北京100050 |
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| 摘 要： | 目的研究BCG-CpG-DNA的免疫学活性。方法通过淋巴细胞增殖、T细胞亚群分析、细胞因子产生、对NK细胞杀伤功能影响等试验,检测BCG-CpG-DNA的免疫活性。结果BCG-CpG-DNA能促进小鼠T、B淋巴细胞增殖,活化巨噬细胞分泌IL-12,并能增强ConA诱导IFN-γ产生,提高小鼠脾细胞中CD3+、CD4+、CD8+T细胞亚群的含量,同时增强小鼠NK细胞的杀伤活性。实验组与对照组所有结果差异均有显著意义(P<0.05)。结论BCG-CpG-DNA能活化抗原呈递细胞(APC),并具有较好的免疫活性。
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| 关 键 词： | BCG-CpG-DNA 佐剂 免疫活性 |
| 收稿时间： | 2006-01-05 |
| 修稿时间： | 2006年1月5日 |
Preliminary Study on Immunological Activity of BCG-CpG-DNA |
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| ZHAO Ai-hua, KOU Li-jie, QIAO Lai-yan, et al.Preliminary Study on Immunological Activity of BCG-CpG-DNA[J].Chinese Journal of Biologicals,2006,19(6):574-577. |
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| Authors: | ZHAO Ai-hua KOU Li-jie QIAO Lai-yan |
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| Affiliation: | National Institute for the Control of Pharmaceutical and Biological Products ,Beijing 100050, China |
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| Abstract: | Objective To study the immunological activity of BCG-CpG-DNA.Methods Determine the immunological activity of BCG-CpG-DNA by lymphocyte proliferation test,T cell subgrouping,the production of cytokine and the effect on killing activity of NK cells.Results BCG-CpG-DNA promoted the proliferation of T and B lymphocytes in mice,activated macrophages to secrete IL-12,enhanced the ability of ConA in inducing IFN-γ,increased the contents of CD3+,CD4+ and CD8+T cell subgroups in murine splenic cells and enhanced the killing activity of murine NK cells.All the results of test and control groups showed significant difference.Conclusion BCG-CpG-DNA activated antigen-presenting cells and showed good immunological activity. |
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| Keywords: | BCG-CpG-DNA Adjuvant Immunological activity |
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