| 转移因子胶囊中多肽与核糖含量测定方法研究 |
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| 引用本文： | 周天琼,俞保彬,倪燕红,周一峰,毛建卫.转移因子胶囊中多肽与核糖含量测定方法研究[J].杭州应用工程技术学院学报,2014(3):172-176. |
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| 作者姓名： | 周天琼 俞保彬 倪燕红 周一峰 毛建卫 |
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| 作者单位： | [1]杭州华津药业股份有限公司,杭州310013 [2]浙江科技学院生物与化学工程学院,杭州310023 [3]浙江省农产品化学与生物加工技术重点实验室,杭州310023 |
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| 基金项目： | 浙江省农业科技成果转化项目（浙科发农[2013]171号-19） |
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| 摘 要： | 研究了转移因子胶囊的多肽与核糖含量测定方法。分别对福林酚法和紫外-可见分光光度法测定转移因子胶囊多肽和核糖含量的方法进行了考察。福林酚法对牛血清白蛋白在30～300μg/m L范围内线性关系良好（r＝0．9990），平均加样回收率为99．77％，RSD为1．31％；紫外-可见分光光度法对D-核糖2～10μg/mL范围内线性关系良好（ r＝0．9997），平均加样回收率99．99％，RSD为1．02％。以上分析方法简便、快速、准确，可作为转移因子胶囊多肽与核糖含量分析的方法。
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| 关 键 词： | 转移因子胶囊 多肽 核糖 含量测定 |
Research on determination for polypeptide and ribose in transfer factor capsules |
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| ZHOU Tianqiong,YU Baobin,NI Yanhong,ZHOU Yifeng,MAO Jianwei.Research on determination for polypeptide and ribose in transfer factor capsules[J].Journal of Hangzhou Institute of Applied Engineering,2014(3):172-176. |
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| Authors: | ZHOU Tianqiong YU Baobin NI Yanhong ZHOU Yifeng MAO Jianwei |
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| Affiliation: | 1. Hangzhou Huajin Pharmaceutical Co. ,Ltd. , Hangzhou 310013, China; 2. School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou 310023, China; 3. Zhejiang Provincial Key Laboratory for Chemical and Biologieal Processing Technology of Farm Produce, Hangzhou 310023, China) |
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| Abstract: | The analytical methods for polypeptide and ribose in transfer factor capsule were studied .The feasibility of Folin phenol method and UV-vis spectrophotometry method for polypeptide and ribose were investigated .The linear range of bovine serum albumin is 30-300μg/mL（r= 0 .999 0） ,and the average recovery of bovine serum albumin is 99 .77% （RSD 1.31% ） .The linear range of D-ribose is 2-10 μg/mL（r=0 .999 7） ,and the average recovery of D-ribose is 99 .99% （RSD 1 .02% ） . The methods reported in this paper are efficient , accurate and suitable for determination for polypeptide and ribose in transfer factor capsules . |
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| Keywords: | transfer factor capsules polypeptide ribose content determination |
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