| 洛伐他汀菌种发酵配方的正交试验 |
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| 引用本文： | 王伟,黄雅琴,李尽哲. 洛伐他汀菌种发酵配方的正交试验[J]. 中国酿造, 2012, 31(3): 39-41 |
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| 作者姓名： | 王伟 黄雅琴 李尽哲 |
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| 作者单位： | 信阳农业高等专科学校 生物技术系,河南信阳,464000 |
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| 基金项目： | 河南省科技计划攻关项目(102102110119) |
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| 摘 要： | 为了确定洛伐他汀发酵培养基的最佳发酵培养基配方,提高发酵水平,先通过单因素试验筛选出最佳碳源和氮源,以及碳源,氮源和无机盐的最佳浓度,再通过正交设计筛选出最佳的发酵培养基配方组合,研究发现土曲霉PF7-15-40的发酵培养基最佳配方是淀粉3.5%,黄豆饼粉4.25%,柠檬酸钠0.10%,磷酸二氢钾2.0g/L,在该条件下洛伐他汀的产量最高。
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| 关 键 词： | 洛伐他汀 土曲霉 发酵 正交试验 |
Optimization of fermentation medium for lovastatin production by orthogonal experiment |
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| WANG Wei , HUANG Yaqin , LI Jinzhe. Optimization of fermentation medium for lovastatin production by orthogonal experiment[J]. China Brewing, 2012, 31(3): 39-41 |
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| Authors: | WANG Wei HUANG Yaqin LI Jinzhe |
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| Affiliation: | (Department of Biotechnology,Xinyang Agricultural College,Xinyang 464000,China) |
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| Abstract: | In order to determine the optimal fermentation medium for lovastatin production by Aspergillus terreus,the optimal concentration of carbon,nitrogen and mineral salt were determined by single-factor test firstly.Then,the optimal fermentation medium was obtained by orthogonal design as followed: starch 3.5%,soybean meal 4.25%,sodium citrate 0.10% and potassium dihydrogen phosphate 2.0g/L.Under these conditions,the highest lovastatin production was obtained. |
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| Keywords: | lovastatin Aspergillus terreus fermentation orthogonal test |
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