|
|||||
|
|
| 双酶法水解双孢蘑菇蛋白的工艺研究 | |
| 引用本文： | 季云琪,王成忠,张绍鹏,徐从勇,张玲.双酶法水解双孢蘑菇蛋白的工艺研究[J].中国调味品,2012,37(4):45-48. |
| 作者姓名： | 季云琪 王成忠 张绍鹏 徐从勇 张玲 |
| 作者单位： | 1. 山东轻工业学院食品科学与生物工程学院,济南,250353 2. 青岛康大食品有限公司,山东青岛,266400 3. 沂水县食品药品监督管理局,山东临沂,276400 |
| 基金项目： | 山东省自然科学基金项目(ZR2011CM039) |
| 摘 要： | 实验研究了纤维素酶和中性蛋白酶共同水解双孢蘑菇蛋白的水解工艺,以α-氨基氮含量为指标,确定了纤维素酶和中性蛋白酶的最佳水解条件为:先加纤维素酶,料液比1∶20,加酶量250 U/g,初始pH值6.0,酶解温度55℃,酶解时间120 min；后加中性蛋白酶,加酶量1500U/g,初始pH值6.5,酶解温度45℃,酶解时间150 min.经双酶水解后,α-氨基氮含量可达36.96mg/g. |
| 关 键 词： | 双孢蘑菇 纤维素酶 蛋白酶 α-氨基氮 |
Technical study of bi-enzyme hydrolysis on Agaricus bisporus protein |
|
| JI Yun-qi , WANG Cheng-zhong , ZHANG Shao-peng , XU Cong-yong , ZHANG Ling.Technical study of bi-enzyme hydrolysis on Agaricus bisporus protein[J].China Condiment,2012,37(4):45-48. | |
| Authors: | JI Yun-qi WANG Cheng-zhong ZHANG Shao-peng XU Cong-yong ZHANG Ling |
| Affiliation: | 1(1.College of Food and Bioengineering,Shan Dong Institute of Light Industry,Ji’nan 250353,China; 2.Qingdao Kangda Foodstuffs Co.,LTD.,Qingdao 266400,China; 3.State Food and Drug Administration of Yishui,Linyi 276400,China) |
| Abstract: | In this study the Agaricus bisporus protein is hydrolyzed by two kinds of enzymes:cellulase and neutral protease.The conditions of extraction are screened with the content of α-amino nitrogen as the marker.The optimum technical conditions are:adding cellulase first,solid to water ratio is 1∶20,amount of cellulase 250 U/g,pH 6.0,temperature 55 ℃ to hydrolyze 120 min,then adding neutral protease,amount of meatral protease 1500 U/g,temperature 45 ℃,pH 6.5 to hydrolyze 150 min.After hydrolyzed by these two enzymes,the content of α-amino nitrogen is 36.96 mg /g. |
| Keywords: | Agaricus bisporus cellulase neutral protease α-amino nitrogen |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |