Detection of tumor markers using single-walled carbon nanotube field effect transistors |
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Authors: | Park Dong-Won Kim Yo-Han Kim Beom Soo So Hye-Mi Won Keehoon Lee Jeong-O Kong Ki-Jeong Chang Hyunju |
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Affiliation: | Department of Chemical Engineering, Chungbuk National University, Cheongju 500-712, Korea. |
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Abstract: | We have developed a biosensor capable of detecting carcinoembryonic antigen (CEA) markers using single-walled carbon nanotube field effect transistors (SWNT-FETs). These SWNT-FETs were fabricated using nanotubes produced by a patterned catalyst growth technique, where the top contact electrodes were generated using conventional photolithography. For biosensor applications, SU-8 negative photoresist patterns were used as an insulation layer. CEA antibodies were employed as recognition elements to specific tumor markers, and were successfully immobilized on the sides of a single-walled carbon nanotube using CDI-Tween 20 linking molecules. The binding of tumor markers to these antibody-functionalized SWNT-FETs was then monitored continuously during exposure to dilute CEA solutions. The observed sharp decrease in conductance demonstrates the possibility of realizing highly sensitive, label-free SWNT-FET-based tumor sensors. |
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