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| 合成血红素加氧酶-1-shRNA载体的实验研究 | |
| 引用本文： | 宋远见,刘红芝,杨冬芝,张璞,刘志安,裴冬生,宗志敏,魏贤勇.合成血红素加氧酶-1-shRNA载体的实验研究[J].广东化工,2011,38(7):21+27-21,27. |
| 作者姓名： | 宋远见 刘红芝 杨冬芝 张璞 刘志安 裴冬生 宗志敏 魏贤勇 |
| 作者单位： | 1. 中国矿业大学,煤炭加工与高效洁净利用教育部重点实验室,江苏,徐州,221116;徐州医学院,神经生物学实验室,江苏,徐州,221002 2. 徐州医学院,神经生物学实验室,江苏,徐州,221002 3. 徐州医学院,肿瘤生物治疗重点实验室,江苏,徐州,221002 4. 中国矿业大学,煤炭加工与高效洁净利用教育部重点实验室,江苏,徐州,221116 |
| 基金项目： | 国家自然科学基金项目，江苏省高校自然科学基金项目，江苏省"青蓝工程"项目资助 |
| 摘 要： | 根据Genebank提供的大鼠HO-1（Hmoxl，血红素加氧酶-1）基因序列，参照RNA干扰序列设计原则，设计针对HO—1的4个RNA干扰序列，定向克隆至表达载体pGPU6-GFP-Neo中，最后采用Baml-I I和PstI酶切凝胶电泳鉴定。鉴定结果显示成功合成了荷载HO-1-shRNA的表达载体。 |
| 关 键 词： | 合成 HO-1基因 shRNA表达质粒 |
Experimental Study about Synthesis of Heme Oxygenase-1-shRNA Vector |
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| Song Yuanjian,Liu Hongzhi,Yang Dongzhi,Zhang Pu,Liu Zhian,Pei Dongsheng,Zong Zhimin,Wei Xianyong.Experimental Study about Synthesis of Heme Oxygenase-1-shRNA Vector[J].Guangdong Chemical Industry,2011,38(7):21+27-21,27. | |
| Authors: | Song Yuanjian Liu Hongzhi Yang Dongzhi Zhang Pu Liu Zhian Pei Dongsheng Zong Zhimin Wei Xianyong |
| Affiliation: | Song Yuanjian1,2,Liu Hongzhi2,Yang Dongzhi2,Zhang Pu2,Liu Zhian2,Pei Dongsheng3,Zong Zhimin1,Wei Xianyong1 (1.Key Laboratory of Coal Processing and Efficient Utilization(Ministry of Education),China University of Mining & Technology,Xuzhou 221008,2.Laboratory of Neurobiology,Xuzhou Medical College,Xuzhou 221002,3.Laboratory of Biological Cancer Therapy,China) |
| Abstract: | Four HO-1 RNA interference sequences were designed according to HO-1(Hmoxl) gene sequences of rats from Genebank with reference to the designing principle of RNA interfering sequences,and were cloned into pGPU6-GFP-Neo expression vector,then were identified by BamH I and Pst I enzyme gel electrophoresis finally.It was proved that we successfully synthesis the expression vector loading HO-1-shRNA. |
| Keywords: | synthesis HO-1 gene shRNA expression plasmid |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |