| Abstract: | Abstract Equilibrium isotherms for adsorption of BSA on DEAE-dextran have been determined experimentally. They were little affected by the initial concentration of BSA but were considerably affected by pH. They were correlated by the Langmuir equation when pH ≥ 5.05 and by the Freundlich equation at pH 4.8 (≈ pI). The maximum amounts of inorganic anion exchanged for BSA were 1 and 0.4% of the exchange capacity of the ion exchanger at pH 6.9 and 4.8, respectively. When NaCl coexisted in the solution, the shape of the isotherm was similar to the Langmuir isotherm but was shifted to the right. When the concentration of NaCl was 200 mol/m3, BSA was not adsorbed on the resin. When BSA was dissolved in pure water, the saturation capacity of BSA on HPO4 2? -form resin was about 3.5 times larger than that with buffer (pH 6.9). The amount of BSA adsorbed on univalent-form resin decreased when the liquid-phase equilibrium concentration of BSA was increased. |
