| Abstract: | For the first time, an efficient purification process of flavonoids and intermediate polar nitrogen-containing constituents from Brassica campestris L. pollen with the help of an improved polystyrene based matrix, LS305 was developed. Three methods (Ultra Fast Liquid Chromatographic with Ultraviolet/Diode Array Detector, spectrophotometric, and automatic Kjeldahl) have been applied for the determination and quantification of flavonoids and nitrogen. With the help of the purification process, the flavonoids content was increased from ～16% to ～45% (w/w). ～6% (w/w) nitrogen originated from intermediate polar nitrogen-containing compounds was retained. These results would contribute to improve industrial processes. |
