2008—2018年四川省阿贡纳沙门菌分子分型及耐药分析

目的 了解2008—2018年四川省阿贡纳沙门菌(Salmonella agona)脉冲场凝胶电泳(PFGE)型别分布和耐药状况,为阿贡纳沙门菌引起的暴发预警、溯源调查及临床治疗提供依据。方法 对经过生化和血清学鉴定的61株阿贡纳沙门菌进行PFGE分子分型分析,采用微量肉汤稀释法测定14种抗生素对菌株的最小抑菌浓度。结果 61株阿贡纳沙门菌分离株共分为41个PFGE型,从不同年份、不同地区临床病例中分离到的部分菌株具有相同的PFGE型别,1株猪肉分离株与部分临床分离株具有相同的PFGE型别。28株阿贡纳沙门菌对14种抗生素均敏感,其余33株菌存在不同程度的耐药,其中四环素耐药率最高,为47.54%(29/61)；多重耐药株共16株,占比为26.23%(16/61)。全部分离株均对亚胺培南敏感。结论 2008—2018年四川省阿贡纳沙门菌分离株PFGE型别呈多样性,在四川省为散发流行状态。耐药情况较严重,耐药性存在上升趋势。

Drug resistance detection and pulsed field gel electrophoresis typing of Salmonella agona in Sichuan Province from 2008 to 2018

Objective In order to discover the pulsed field gel electrophoresis (PFGE) serotypes and drug resistance of Salmonella agona (S. agona) in Sichuan Province during 2008 to 2018, so as to understand the contamination status of S. agona and provide reference data for outbreak warning, traceability investigation and antibiotic use strategy. Methods A total of 61 S. agona strains after the serological and biochemical identification were analyzed by PFGE and tested for minimal inhibitory concentration of 14 antibiotics. Results The isolates of S. agona were divided into 41 PFGE types, some strains isolated from clinical cases in different years and regions had the same PFGE type, one pork isolate had the same PFGE type as some clinical isolates. The antibiotic resistance test indicated 28 of the isolates were sensitive to 14 kinds of antibiotics, the other 33 strains showed different degrees of drug resistance, nearly half of the strains were insensitive to tetracycline; and there were 16 multidrug-resistant strains. All of the isolates were sensitive to imipenem. Conclusion The PFGE types of S. agona isolated in Sichuan from 2008 to 2018 showed diversity, and they were sporadic prevalent in Sichuan Province. The drug resistance was serious with an increasing trend.