| 三聚氰胺人工抗原及多克隆抗体的制备 |
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| 引用本文： | 龚云飞,王唯芬,张明洲,陈宗伦,彭雪,李沐洁,奚茜.三聚氰胺人工抗原及多克隆抗体的制备[J].中国计量学院学报,2011,22(1):73-79. |
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| 作者姓名： | 龚云飞 王唯芬 张明洲 陈宗伦 彭雪 李沐洁 奚茜 |
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| 作者单位： | 中国计量学院,生命科学学院,浙江,杭州,310018 |
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| 基金项目： | 浙江省重大科技专项，杭州市重大科技创新专项，浙江省大学生科技创新项目 |
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| 摘 要： | 采用戊二醛法将三聚氰胺(Melamine,MEL)偶联到牛血清白蛋白(BSA)上合成免疫原三聚氰胺-牛血清白蛋白(MEL-BSA).三聚氰胺甲醛树脂法对三聚氰胺衍生化,再偶联到鸡卵清蛋白(OVA)合成包被原三聚氰胺-鸡卵清蛋白(MEL-OVA).对纯化后的合成抗原进行紫外扫描、红外扫描和SDS-PAGE电泳鉴定.免疫新西兰大白兔,检测抗体的生成.结果表明,免疫兔血清的效价达到了1∶20 000,从而为进一步建立三聚氰胺的免疫检测方法奠定了基础.
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| 关 键 词： | 三聚氰胺 人工抗原 多克隆抗体 |
Preparation of artificial antigen of melamine and polyclonal antibody |
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| GONG Yun-fei,WANG Wei-fen,ZHANG Ming-zhou,CHEN Zong-lun,PENG Xue,LI Mu-jie,XI Xi.Preparation of artificial antigen of melamine and polyclonal antibody[J].Journal of China Jiliang University,2011,22(1):73-79. |
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| Authors: | GONG Yun-fei WANG Wei-fen ZHANG Ming-zhou CHEN Zong-lun PENG Xue LI Mu-jie XI Xi |
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| Affiliation: | GONG Yun-fei; WANG Wei-fen; ZHANG Ming-zhou; CHEN Zong-lun; PENG Xue; LI Mu-jie; XI Xi(College of Life Science; China Jiliang University; Hangzhou 310018; China); |
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| Abstract: | Through the method of glutaraldehyde,the melamine(MEL) was coupled to the carrier protein for synthesizing the artificial antigen.Melamine was linked to bovine serum ovabumin(BSA) to generate immunogenic antigen(MEL-BSA) and was linked to ovabumin(OVA) by melamino-formaldehyde resin method to generate coating antigen(MEL-OVA).Artificial antigen was purified by FPLC,and then identified by UV and IR as well as SDS-PAGE.Mel-BSA was used to immune New Zealand white rabbits,and the generation of antibody was det... |
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| Keywords: | melamine artifical antigen polyclonal antibody |
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