| 茭白黑粉菌核相变化及ISSR分子标记筛选的研究 |
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| 引用本文： | 赵煦泓,尤文雨,崔海峰,俞晓平,叶子弘.茭白黑粉菌核相变化及ISSR分子标记筛选的研究[J].中国计量学院学报,2011,22(2):176-180. |
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| 作者姓名： | 赵煦泓 尤文雨 崔海峰 俞晓平 叶子弘 |
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| 作者单位： | 中国计量学院生命科学学院,浙江省生物计量与检验检疫技术重点实验室,浙江杭州310018 |
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| 基金项目： | 国家自然科学基金，浙江省自然科学基金，浙江省科技计划重大项目 |
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| 摘 要： | 通过显微观察和Hoechst 33258荧光染色方法,系统观察了茭白灰茭中黑粉菌厚垣孢子不同萌发时期的核相变化.研究表明,厚垣孢子萌发过程中黑粉菌细胞核的数目不断变化,核相在萌发过程中是不规则的.茭白灰茭厚垣孢子中没有观察到细胞核,菌丝体细胞核的数目在1~4个,大多数小孢子仅有一个细胞核或没有细胞核.同时获得了18个可用于茭白黑粉菌遗传变异分析的ISSR分子标记,作为深入探讨茭白黑粉菌的生物学特性的参考.
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| 关 键 词： | 茭白 黑粉菌 核相 分子标记 |
Studies on changes in nuclear conditions and molecule marker selection of polymorphism in Ustilago esculenta |
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| ZHAO Xun-hong,YOU Wen-yu,CUI Hai-feng,YU Xiao-ping,YE Zi-hong.Studies on changes in nuclear conditions and molecule marker selection of polymorphism in Ustilago esculenta[J].Journal of China Jiliang University,2011,22(2):176-180. |
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| Authors: | ZHAO Xun-hong YOU Wen-yu CUI Hai-feng YU Xiao-ping YE Zi-hong |
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| Affiliation: | ZHAO Xun-hong,YOU Wen-yu,CUI Hai-feng,YU Xiao-ping,YE Zi-hong(Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine Technique,College of life science,China Jiliang University,Hangzhou 310018,China) |
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| Abstract: | Using the fluorescence dye Hoechst 33258,nuclear condition of Ustilago esculenta was detected by the fluorescence microscopy during the different stages of chlamydospores bourgeoning.It was found that the numbers of nucleus were continually changed irregularly.The mubers of nucleus in mycelium were 1 to 4,and no more than 1 nuclear were detected in most of the microspores.There is no nuclear fluorescence in chlamydospores.Besides,the polymorphism of Ustilago esculenta were analysed by ISSR molecule markers.... |
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| Keywords: | Zizania latifolia Ustilago esculenta nuclear condition molecule markers |
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