Effects of the age on the apoptotic and proliferative reactions in the constant light-exposed rat retina

The effects of age (5-3 weeks old) on apoptotic changes in the rat photoreceptor cells induced by 3 days of constant light exposure were examined using TUNEL (TdT-mediated dUTP nick end labeling). The effects on the expression of the Ki67-antigen, which is a proliferative marker, in these photoreceptor cells were also examined by immunohistochemistry. The results suggested that the number of positive cells in the outer nuclear layer of the superior hemisphere is higher than in the inferior nuclear layer in both the TUNEL reaction and the distribution of the Ki67 antigen, and that the number of positive cells increases with age in general. The cells of monocytes/macrophages may locally proliferate in the retina to phagocytose the apoptotic bodies owing to the degeneration of photoreceptor cells. The present findings revealed that the rates of these reactions may generally increase with age.