利用廉价原料生产聚谷氨酸的研究

目的 降低聚谷氨酸的生产成本.方法 从聚谷氨酸发酵所需的氮源和碳源出发,分别研究了小麦水解蛋白、豆饼粉、豆粕粉、玉米蛋白、玉米浆等有机氮源和甘蔗糖蜜、甜菜糖蜜、葡萄糖蜜、玉米糖蜜等碳源对聚谷氨酸发酵产量的影响,在此基础上通过单因素实验优化了玉米浆、硫酸铵、甜菜蜜、谷氨酸钠的添加量,最后通过发酵罐发酵比较了优化后的工艺与...

Study on Production of Polyglutamic Acid from Cheap Raw Materials

Objective To reduce the production cost of γ-polyglutamic acid.Methods Based on the nitrogen and carbon sources for polyglutamic acid fermentation,in this paper,the effects of organic nitrogen sources(such as wheat hydrolyzed protein,soybean cake powder,soybean meal powder,corn protein,corn pulp)and carbon sources(such as sugarcane molasses,beet molasses,grape molasses and corn molasses)on polyglutamic acid fermentation yield were studied respectively.On this basis,the addition amounts of corn pulp,ammonium sulfate,beet honey and monosodium glutamate were optimized by a single factor experiment.Finally,the optimized fermentation process was compared with that using peptone and glucose as nitrogen and carbon sources.Results Under the conditions of corn pulp 40 g/L,ammonium sulfate 5g/L,sugar beet honey 350 g/L and sodium glutamate 70 g/L,the yield of γ-polyglutamic acid reached 45.34 g/L,which was higher than 40.46 g/L of the fermentation level of peptone and glucose.Conclusion By using cheap nitrogen source corn pulp,ammonium sulfate and carbon source beet honey,γ-polyglutamic acid can be produced at low cost,the yield is increased by 12%and the production cost is reduced by more than 20%.