Alterations in cell cycle regulation in mouse skin tumors |
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Authors: | S Balasubramanian N Ahmad S Jeedigunta H Mukhtar |
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Affiliation: | Laboratory of Biodynamics, Tokyo Institute of Technology, Yokohama, Japan. |
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Abstract: | Using a modified tip of the atomic force microscope (AFM), we harvested several strands of genomic DNA from a nanometer region of mouse chromosomes. We have also co-developed a random PCR method to amplify the recovered genomic DNA, in which a single DNA molecule of several kilobasepairs could be amplified efficiently. A subsequent fluorescence in situ hybridization (FISH) indicated that the amplified DNA originally came from the tip-manipulated regions of mouse chromosomes. Several fragments containing unique sequences were identified using Southern hybridization after subcloning the PCR products into pUC18 plasmid. The present results showed a potential application of AFM to genomic analysis. |
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