| 芦丁-α-鼠李糖苷酶基因的克隆 |
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| 引用本文： | 马安周,王栩林,鱼红闪,金凤燮. 芦丁-α-鼠李糖苷酶基因的克隆[J]. 大连工业大学学报, 2005, 24(1) |
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| 作者姓名： | 马安周 王栩林 鱼红闪 金凤燮 |
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| 摘 要： | 根据芦丁 α 鼠李糖苷酶N端氨基酸序列设计简并引物,从Absidiasp.R9g的总RNA出发,通过逆转录 聚合酶链反应(RT PCR)扩增出芦丁 α 鼠李糖苷酶基因片段。将RT PCR获得的1条1.6kb的目的条带与pMD18 T载体连接,转化大肠杆菌JM109感受态细胞。酶切鉴定结果表明目的片段克隆成功。
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| 关 键 词： | 芦丁-α-鼠李糖苷酶 RT-PCR 克隆 |
Cloning of rutin-α-rhamnosidase gene |
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| MA An-zhou,WANG Xu-lin,YU Hong-shan,JIN Feng-xie. Cloning of rutin-α-rhamnosidase gene[J]. Journal of Dalian Dalian Polytechnic University, 2005, 24(1) |
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| Authors: | MA An-zhou WANG Xu-lin YU Hong-shan JIN Feng-xie |
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| Abstract: | RT-PCR was carried out with degenerate primers designed according to the N-terminal amino acid sequences of rutin-α-rhamnosidase. A 1.6?kb cDNA fragment was amplified from the total RNA of Absidia sp.R9g and ligated with vector pMD18-T.E.coli JM109 competent cells were transformed with the recombinant plasmid. The result shows that clones are successful. |
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| Keywords: | RT-PCR |
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