Silver ion high-performance liquid chromatography of the triacylglycerols ofCrepis alpina seed oil |
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Authors: | W E Neff R O Adlof M El-Agaimy |
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Affiliation: | (1) Present address: Quality and Safety Research, NCAUR, ARS, USDA, 1815 N. University Street, 61604 Peoria, IL;(2) Oils and Fats Research Section, Food Technology Research Institute, Agricultural Research Center, Giza, Egypt |
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Abstract: | The triacylglycerols ofCrepis alpina oil were characterized because this oil has a high concentration of crepenynic (cis-9-octadecen-12-ynoic) acid, a fatty acid useful in the chemical synthesis of deuterated fats for human metabolism studies.
The triacylglycerols were separated from the crude oil by solid-phase extraction. Resolution, quantitation and isolation of
the individual triacylglycerols were performed by silver ion high-performance liquid chromatography on a commercial column,
an acetonitrile in hexane isocratic mobile phase and flame-ionization detection. Isolated triacylglycerols were identified
by capillary gas chromatography of their fatty acid methyl esters. Of the eleven eluted triacylglycerols ofCrepis alpina oil, 85% included 35% tricrepenynoyl, 34% linoleoyldicrepenynoyl and 16% dilinoleoylcrepenynoyl glycerols. Triacylglycerols
eluted according to the numbers of alkene and alkyne bonds. Elution times, resolution and quantitation were reproducible over
a three-month period. The flame-ionization detector response required no response factors for quantitation of the triacylglycerols
present inCrepis alpina oil. The silver ion chromatography system permitted the identification of 95% of the triacylglycerols compared to 70% of
the triacylglycerols previously identified with reversed-phase high-performance liquid chromatography. |
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Keywords: | Alkynoic acids crepenynic acid Crepis alpina oil flame-ionization detection silver ion high-performance liquid chromatography triacylglycerols tricrepenynoyl glycerol |
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