Two-step fermentation process and the corresponding study on its enzymology characteristics

Strain HIT-3, which has good performances in cellulose degradation, was isolated from the campus soil. Based on the identification of conservative sequences 16S rDNA and the analysis of physiological-biochemical characteristics, HIT-3 was identified as Achromobacter xylosoxidans. Denitrificans. A two-step fermentation process was conducted by adopting compound-bioflocculant-producing flora constructed by cellulose-degrading bacterium HIT-3 and flocculating bacterium F2. The cellulose degradation metabolites of HIT-3 was taken as substrates by flocculating bacterium F2, by which excellent compound bio-flocculant was obtained. In addition, the enzymology characteristics of HIT-3 were investigated when cultured in cellulose media, which utilized CMC-Na as its sole carbon. The results show that HIT-3 achieves a climax of 67. 6 U/mL of enzyme production after incubation for 6 d, and the organic carbons produced are sufficient as the substrates required by the fermentation of flocculating bacterium F2 (flocculating efficiency of 85.6%), which makes it feasible to reuse bioenergy.