Effect of shear stress on the growth of continuous culture of Synechocystis PCC 6803 in a flat-panel photobioreactor |
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Authors: | Min-Gyu Sung Won-Sub Shin Woong Kim Jong-Hee Kwon Ji-Won Yang |
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Affiliation: | 1. Department of Chemical and Biomolecular Engineering, KAIST, Yuseong-gu, Daejeon, 305-701, Korea 2. Plant Biochemistry, Faculty of Biology & Biotechnology, Ruhr University Bochum, D-44780, Bochum, Germany 3. Advanced Biomass R&D Center, KAIST, Yuseong-gu, Daejeon, 305-701, Korea
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Abstract: | The effect of hydrodynamic forces generated by air bubbles on cell growth of continuous culture of Synechocystis PCC 6803 was studied in a flat-panel photobioreactor. Keeping all relevant parameters constant enables the optimization of individual parameters, for which a continuous cultivation approach has significant advantages. Continuous culture of Synechocystis PCC 6803 was cultivated under different gas velocities from 0.022 m s?1 up to 0.128 m s?1. Based on direct determination of effective growth rate at constant cell densities, cell damage due to shear stress induced by the increasing gas velocity at the sparger was directly observed. A significant decrease of effective growth rate was observed at gas velocity of 0.085 m s?1 generated at the gas flow rate of 200 ml min?1, indicating cell damage by shear stress. Optimization of gas volume and the development of an effective aeration system corresponding to a given reactor setup is important to realize a reliable cell growth. |
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