| 液相色谱法与氨基酸分析仪法测定人乳中 水解氨基酸的比较研究 |
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| 引用本文： | 李 爽,陈 启,蔡明明,任一平.液相色谱法与氨基酸分析仪法测定人乳中 水解氨基酸的比较研究[J].食品安全质量检测技术,2014,5(7):2073-2079. |
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| 作者姓名： | 李 爽 陈 启 蔡明明 任一平 |
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| 作者单位： | 贝因美婴童食品股份有限公司,贝因美婴童食品股份有限公司,贝因美婴童食品股份有限公司,浙江省疾病预防控制中心 |
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| 摘 要： | 目的：研究氨基酸自动分析仪法和液相色谱法测定母乳中水解氨基酸含量的比较。方法：采用液相色谱柱前衍生和氨基酸分析仪测定母乳中水解氨基酸,液相色谱法选用C18 柱和AQC进行衍生,氨基酸分析仪采用阳离子交换柱和柱后茚三酮衍生。结果：两种方法的方法学参数无明显差异,所测结果差异均在4.65 %以下。结论：两种方法均可采用,可作为互相验证的方法。
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| 关 键 词： | 水解氨基酸 衍生 液相色谱 氨基酸分析仪 |
| 收稿时间： | 2014/6/11 0:00:00 |
| 修稿时间： | 2014/7/14 0:00:00 |
Comparative study on quantification of hydrolysis amino acids in human milk using liquid chromatography and amino acid analyzer |
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| LI Shuang,CHEN Qi,CAI Ming-Ming and REN Yi-Ping.Comparative study on quantification of hydrolysis amino acids in human milk using liquid chromatography and amino acid analyzer[J].Food Safety and Quality Detection Technology,2014,5(7):2073-2079. |
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| Authors: | LI Shuang CHEN Qi CAI Ming-Ming and REN Yi-Ping |
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| Affiliation: | Beingmate Baby Child Food Co,Ltd,Beingmate Baby Child Food Co,Ltd,Beingmate Baby Child Food Co,Ltd,Zhejiang Provincial Center for Disease Prevention and Control |
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| Abstract: | Objective Comparison the methods of amino acid analyzer (AAA) and ultra-performance liquid chromatography (UPLC) for determination of hydrolysis amino acids in human milk. Methods Hydrolysis amino acids were analysed with C18 column and AQC precolumn derivation by UPLC method; and ion ex-change chromatography column and ninhydrinpostcolumn derivation by AAA method. Results Two methods provided similar validation results. The difference of analysis result using two methods was below 4.65%. Conclusion Both methods can be used for the quantitation of hydrolysis amino acids. |
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| Keywords: | Hydrolysis Amino Acid Derivative Ultra-Performance Liquid Chromatography Amino Acid Analyzer |
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