| 99Tcm-MAG3-Lys-mPEG的制备及其在小鼠体内的分布 |
| |
| 引用本文： | 付成锋,杨顺龙,王毅,刘新起,褚泰伟,胡少文,王祥云. 99Tcm-MAG3-Lys-mPEG的制备及其在小鼠体内的分布[J]. 同位素, 2005, 18(3): 142-147 |
| |
| 作者姓名： | 付成锋 杨顺龙 王毅 刘新起 褚泰伟 胡少文 王祥云 |
| |
| 作者单位： | 北京大学,化学与分子工程学院,应用化学系,北京,100871 |
| |
| 基金项目： | 国家自然科学基金资助课题（B0109） |
| |
| 摘 要： | 以mPEG-5000、Fmoe-Lysine和MAG3为原料，经过五步反应合成了载体MAG3-Lys-mPEG；对其进行^99Tc^m标记后，进一步研究了标记物的体外稳定性及其在小鼠体内的生物分布。结果表明，^99Tc^m-MAG3-Lys-mPEG的标记率〉98％；标记物在体外有很好的稳定性，在生理盐水中放置24h时，放化纯度〉91％；在血清中温浴16．5h时放化纯度仍〉92％；标记物在小鼠体内的血液清除较快，在肾脏中的摄取最高，标记物主要经肾脏通过尿液排出体外。mPEG有可能用作反义核酸的有效载体。
|
| 关 键 词： | ^99Tc^m mPEG MAG3-Lys-mPEG 生物分布 |
| 文章编号： | 1000-7512（2005）03-0142-06 |
| 收稿时间： | 2004-08-31 |
| 修稿时间： | 2005-07-10 |
Preparation and Biodistribution of 99Tcm-MAG3-Lys-mPEG |
| |
| FU Cheng-feng,YANG Shun-long,WANG Yi,LIU Xin-qi,CHU Tai-wei,HU Shao-wen,WANG Xiang-yun. Preparation and Biodistribution of 99Tcm-MAG3-Lys-mPEG[J]. Isotopes, 2005, 18(3): 142-147 |
| |
| Authors: | FU Cheng-feng YANG Shun-long WANG Yi LIU Xin-qi CHU Tai-wei HU Shao-wen WANG Xiang-yun |
| |
| Affiliation: | Department of Applied Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China |
| |
| Abstract: | Starting with mPEG-5000,Fmoc-Lysine,and mercaptoacetyltriglycine(MAG_3),the carrier MAG_3-Lys-mPEG is synthesized through five steps,which carrier is labeled by ~(99)Tc~m with labeling efficiency >98%.~(99)Tc~m-MAG_3-Lys-mPEG shows high stability in vitro.The radiochemical purity keeps up >91% and >92% after incubation in saline for 24 h and in bovine serum for 16.5 h,respectively.The biodistribution in mice indicates that ~(99)Tc~m-MAG_3-Lys-mPEG is quickly cleared from blood,and excreted mainly through the urinary systemr.There is the highest uptake in kidneys.It is expected that mPEG may be used as an effective ASODN carrier. |
| |
| Keywords: | ^99Tc^m mPEG MAGa-Lys-mPEG biodistribution |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
