| 呋喃丹和氯氰菊酯的类雌激素活性研究 |
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| 引用本文： | 郭佳,杨雪,张灿,何桢,钟秋,熊丽,刘德立. 呋喃丹和氯氰菊酯的类雌激素活性研究[J]. 农药, 2012, 51(2): 105-107,150 |
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| 作者姓名： | 郭佳 杨雪 张灿 何桢 钟秋 熊丽 刘德立 |
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| 作者单位： | 华中师范大学生命科学学院湖北省遗传调控与整合生物学重点实验室,武汉,430079 |
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| 基金项目： | 国家自然科学基金(30771429,31071653);教育部留学归国人员科研基金 |
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| 摘 要： | [目的]探究呋喃丹和氯氰菊酯的类雌激素活性。[方法]采用重组人雌激素受体α基因的双杂交酵母测定了17β-雌二醇和2种农药诱导产生β-半乳糖苷酶活性的EC50值。[结果]17β-雌二醇、呋喃丹、氯氰菊酯对应的EC50值分别为4.04×10-10、2.34×10-7、1.04×10-6mol/L,诱导的最大酶活分别为4.97、1.82、1.59 U。[结论]2种农药均具有不同程度的类雌激素活性,且呋喃丹的类雌激素活性高于氯氰菊酯。
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| 关 键 词： | 重组基因酵母 类雌激素活性 氯氰菊酯 呋喃丹 |
Exploring the Estrogenicity of Carbofuran and Cypermethrin |
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| GUO Jia , YANG Xue , ZHANG Can , HE Zhen , ZHONG Qiu , XIONG Li , LIU De-li. Exploring the Estrogenicity of Carbofuran and Cypermethrin[J]. Pesticides, 2012, 51(2): 105-107,150 |
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| Authors: | GUO Jia YANG Xue ZHANG Can HE Zhen ZHONG Qiu XIONG Li LIU De-li |
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| Affiliation: | (Hubei Key Laboratory of Genetic Regulation and Integrative Biology,College of Life Science,Central China Normal University,Wuhan 430079,China) |
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| Abstract: | the aim is to explore the estrogenicity of carbofuran and cypermethrin.[Methods] The EC50 values of the activity of β-galactosidase induced by 17β-estradiol,carbofuran and cypermethrin by recombining human estrogen receptor α gene yeast was determined.[Results] The EC50 values of 17β-estradiol,carbofuran and cypermethrin are 4.04×10-10,2.34×10-7,1.04×10-6 mol/L respectively and the maximum enzyme activities are 4.97,1.82 and 1.59 U respectively.[Conclusions] Carbofuran and cypermethrin have different levels of estrogenicity while carbofuran is shown to have higher estrogenicity. |
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| Keywords: | recombinant yeast estrogenicity cypermethrin carbofuran |
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