白腐菌漆酶对莠去津最适降解条件的探讨

目的]将白腐菌Coprinopsis cinerea okayama 7#130漆酶对三嗪类农药莠去津的降解条件进行了研究。方法]向纯漆酶溶液中加入一定浓度的莠去津溶液进行降解反应,采用HPLC测定其含量,计算降解率。利用Box-Behnken设计对影响降解此农药的关键因素进行优化。结果]经Design-Expert软件二次回归分析,得到莠去津降解的最适条件:pH值为6.9,温度为38.3℃,反应体系体积为5.3 mL,转速为186 r/min。在此条件下,莠去津降解率响应预测值为52.41%,验证值为52.32%。结论]最适条件下的降解率提高了10%~15%,这为降低莠去津对环境的危害提供了科学依据。

Research on the Optimum Degradation Conditions of Atrazine by White Rot Fungi Laccase

Aims] The degradation conditions of triazine pesticide atrazine by white-rot fungi Coprinopsis cinerea okayama7# 130 laccase were studied.Methods] Some certain concentration of atrazine was added to the pure laccase solution before the degradation.The HPLC was performed to determine the content,and then the degradation rate was calculated.The key factors influencing the degradation of atrazine were optimized by Box-Behnken design.Results] The result was undertaken by the second regression analysis using Design-Expert software,which showed that the optimal condition of the atrazine degrading was as follows: The pH value was 6.9,the temperature was 38.3 °C,the reaction volume was 5.3 mL,and the shaking speed was 186 r/min respectively.Under the optimal condition,the predicted value of the atrazine degradation rate was 52.41%,and the experimental value of atrazine degradation rate was 52.32%.Conclusions] The degradation rate increased by 10-15% under the optimal condition,which provided the scientific basis to reduce the harm to the environment caused by atrazine.