Rapid Screening of Lectins for Multivalency Effects with a Glycodendrimer Microarray |
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| Authors: | Núria Parera Pera Dr. Hilbert M. Branderhorst Dr. Raymond Kooij Caroline Maierhofer Dr. Marjolein van der Kaaden Rob M. J. Liskamp Prof. Dr. Valentin Wittmann Prof. Dr. Rob Ruijtenbeek Dr. Roland J. Pieters Dr. |
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| Affiliation: | 1. Department of Medicinal Chemistry and Chemical Biology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, P.?O. Box 80082, 3508?TB Utrecht (The Netherlands), Fax: (+31)?30‐2536944;2. Universit?t Konstanz, Fachbereich Chemie, Fach 709, 78457 Konstanz (Germany);3. Pamgene International B.V. P.?O. Box 1335, 5200?BJ 's‐Hertogenbosch (The Netherlands) |
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| Abstract: | Multivalency is an important phenomenon in protein–carbohydrate interactions. In order to evaluate glycodendrimers as multivalent inhibitors of carbohydrate binding proteins, we displayed them on a microarray surface. Valencies were varied from 1 to 8, and corrections were made for the valencies so that all surfaces contained the same amount of the sugar ligand. Five different carbohydrates were attached to the dendrimers. A series of fluorescent lectins was evaluated, and for each of them a binding profile was obtained from a single experiment showing both the specificity of the lectin for a certain sugar and whether it prefers multivalent ligands or not. Very distinct binding patterns were seen for the various lectins. The results were rationalized with respect to the interbinding distances of the lectins. |
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| Keywords: | carbohydrates click chemistry dendrimers inhibitors microarrays |
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