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Solution Structure of the Leader Sequence of the Patellamide Precursor Peptide,PatE1–34 |
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| Authors: | Wael E Houssen Dr Stephen H Wright Dr Arnout P Kalverda Dr Gary S Thompson Dr Sharon M Kelly Dr Marcel Jaspars Prof |
| Affiliation: | 1. Marine Biodiscovery Centre, Department of Chemistry, University of Aberdeen, Aberdeen AB24 3UE, Scotland (UK), Fax: (+44)?1224‐272921;2. Astbury Centre for Structural Molecular Biology, Faculty of Biological Sciences, University of Leeds, Leeds LS2 9JT (UK);3. Division of Molecular and Cellular Biology, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow GI2 8QQ (UK) |
| Abstract: | The solution structure of the leader sequence of the patellamide precursor peptide was analysed by using CD and determined with NOE‐restrained molecular dynamics calculations. This leader sequence is highly conserved in the precursor peptides of some other cyanobactins harbouring heterocycles, and is assumed to play a role in targeting the precursor peptide to the post‐translational machinery. The sequence was observed to form an α‐helix spanning residues 13–28 with a hydrophobic surface on one side of the helix. This hydrophobic surface is proposed to be the site of the initial binding with modifying enzymes. |
| Keywords: | biosynthesis cyanobactins patellamides peptides Prochloron |