海参酶解物促SD大鼠皮肤创面愈合效果的研究

目的:为探究海参酶解物(sea cucumber enzymatic hydrolysate,SCH)对SD大鼠皮肤创面愈合的影响,为SCH应用于创面愈合提供理论依据.方法:设立模型组、3个SCH剂量组和阳性组,建立大鼠全层皮肤切除模型,于术后第4、8和12天分批处死.通过计算创面愈合率、进行组织切片伊红染色(hema...

Effect of sea cucumber enzymatic hydrolysate on skin wound healing in SD rats

The impact of sea cucumber hydrolysate(SCH)on skin wound healing in SD rats was investigated,aiming to provide basis for the application of SCH in wound healing.A rat model of full-thickness cutaneous wounds was established in a model group,three SCH dose groups and a positive group,and the rats were sacrificed in batches on the 4 th,8 th and 12 nd day after surgery.The effect of SCH on wound healing was comprehensively analyzed in combination with the results of wound healing rate,observation of tissues stained with hematoxylin-eosin(H&E)and Masson’s trichrome method(Masson),changes of interleukin-8(IL-8)and interleukin-10(IL-10)in the blood,detection of hydroxyproline(Hyp),vascular endothelial growth factor(VEGF),epidermal growth factor(EGF)and basic fibroblast growth factor(bFGF)in wounds.Results showed that the wound healing rate of each dose group of SCH was faster than that of the model group.H&E results showed that SCH could regulate the infiltration of inflammatory cells and promote angiogenesis,and the epidermis recovered fastest in the medium dose group.Based on the results of IL-8 and IL-10,SCH could regulate their secretion to reduce the inflammatory reaction duration.Collagen fibers formation in the wound significantly promoted by SCH was observed in Masson’s trichrome stained tissues,and Hyp content increased accordingly.In addition,the mechanism of SCH wound healing may be related to the significant increase of growth factors secretion.The prepared SCH could effectively promote wound healing in SD rats,with best performance observed in the medium dose group(0.50 g/kg).