微生物快速检测用生物传感器基本特性研究

针对食品卫生现场检测需求,基于三磷酸腺苷(ATP)生物发光原理设计了一种新型的微生物快速检测用生物传感器,并以ATP标准品为检测对象,结合荧光分光光度计对传感器的响应波长、检测时间及响应范围等基本特性进行了初步研究。研究结果表明,该传感器对标准ATP响应迅速,其光学响应波长为550nm;在1~100nmol/L ATP浓度范围内,传感器响应光强信号与ATP浓度之间呈现出良好的正响应关系。此外,通过如加入一定CoA等方式来优化检测试剂配方,可以进一步来改进传感器的检测灵敏度。该传感器在结构上采用集成化设计,具有无需微生物培养、操作简单及响应快速的优点,在食品工业领域快速检测物体表面微生物污染方面具有广泛应用前景。

Study on the Response Characteristics of Biosensor for Rapid Detection of Microbe

A new biosensor for rapid detection of microorganism was designed to meet the demand of instantly evaluating food quality and sanitation in most enterprises.It was based on ATP bioluminescence technology and integrated the sampling,the ATP extraction and the measuring part in its configuration.And the basic response characteristics of the biosensor were primarily investigated through experiments.The results show that its response wavelength is about 550 nm.When detected at this wavelength,it answers rapidly to the standard ATP to generate fluorescence.And the fluorescence intensity correlates well with ATP concentration in the range of 1~100 nmol/L.Moreover,the sensitivity of the biosensor could be improved by the optimization of its sensitive reagent such as adding a certain Coenzyme A.In summary,the biosensor has virtue of fast response,convenient to operate and has a potential to be applied to rapidly evaluate microbe contamination in surfaces in situ in food industry.