| 樱桃番茄叶体细胞胚发生的组织细胞学观察 |
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| 引用本文： | 关正君,郭斌,尉亚辉.樱桃番茄叶体细胞胚发生的组织细胞学观察[J].电子显微学报,2011,30(2):158-165. |
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| 作者姓名： | 关正君 郭斌 尉亚辉 |
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| 作者单位： | 关正君,GUAN Zheng-jun(运城学院生命科学系,山西运城044000;西北大学生命科学学院西部资源生物与现代生物技术省部共建教育重点实验室,陕西,西安,710069);郭斌,尉亚辉,GUO Bin,WEI Ya-hui(运城学院生命科学系,山西运城,044000) |
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| 基金项目： | 国家自然科学基金资助项目，陕西省生物技术重点实验室项目，陕西省教育厅专项科研资助项目，陕西省财政专项，西北大学自主创新类项目 |
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| 摘 要： | 以樱桃番茄无菌苗叶片为外植体,在附加2.0 mg/L 6-BA+0.1 mg/L IAA的MS培养基上诱导培养,利用石蜡切片和透射电镜技术观察外植体体细胞胚发生过程中显微结构和超微结构的变化.显微观察结果表明,培养5天后,部分叶肉细胞通过脱分化而进入分裂,开始形成胚性细胞;13天后切口处可诱导出淡黄色胚性愈伤组织,继续...
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| 关 键 词： | 樱桃番茄 体细胞胚发生 显微结构 超微结构 |
Histocytological observation on somatic embryogenesis derived from leaves of cherry tomato |
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| GUAN Zheng-jun,GUO Bin,WEI Ya-hui.Histocytological observation on somatic embryogenesis derived from leaves of cherry tomato[J].Journal of Chinese Electron Microscopy Society,2011,30(2):158-165. |
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| Authors: | GUAN Zheng-jun GUO Bin WEI Ya-hui |
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| Affiliation: | GUAN Zheng-jun1,2,GUO Bin1,WEI Ya-hui1(1.Department of Life Sciences,Yuncheng University,Yuncheng Shanxi 044000,2.Key Laboratory of Resource Biologyand Biotechnology in Western China,Ministry of Education,Northwest University,Xi'an Shaanxi 710069,China) |
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| Abstract: | The leaves of cherry tomato（Lycopersivon esculentum Mill.） as explants were cultured on MS medium added with 6-BA 2.0 mg·L-1 and IAA 0.1 mg·L-1 to form embryogenetic callus.The microstructure and ultrastructure of the explants were observed by paraffin sections and transmission electron microscopy during somatic embryogenesis.The results showed that some of the mesophyll cells went into dividing through dedifferentiation and started to form embryonic cells at the cultured 5th day.The incision could be induced as light yellow embryogenic callus after 13 days and could continue to produce somatic embryos.Somatic embryogenesis was not synchronized,and went through globular stage,heart-shaped stage,torpedo stage and cotyledon stage,and at the same time had the appearance of abnormal embryos.The emergence of secondary embryos accompanied by the radicle further development.Ultrastructural observation showed that the cytoplasm,nucleus,cell wall,mitochondria,Golgi bodies,chloroplast and other structures in embryonic cells of cherry tomato explants changed regularly during in vitro somatic embryogenesis. |
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| Keywords: | cherry tomato somatic embryogenesis histocytology ultrastructure |
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