| 新筛选菌种Sphingopyxis sp.USTB-05对微囊藻毒素的生物降解 |
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| 引用本文： | 王俊峰,吴鹏飞,闫海,陈建.新筛选菌种Sphingopyxis sp.USTB-05对微囊藻毒素的生物降解[J].中国化学工程学报,2010,18(1):108-112. |
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| 作者姓名： | 王俊峰 吴鹏飞 闫海 陈建 |
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| 作者单位： | |
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| 基金项目： | 国家重点基础研究发展计划(973计划)，the National Natural Sci-ence Foundation of China，the State Key Joint Laboratory of Environment Simulation and Pollution Control，the Educational Committee of Beijing |
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| 摘 要： | A promising bacterial strain for the biodegradation of Microcystins (MCs) was isolated from Dianchi lake in China and identified as Sphingopyxis sp. USTB-05 by the analysis of 16s rDNA. Initial MC-RR of 42.3 mg&;#8226;L－1 was completely degraded by USTB-05 within 36 h, which was a relatively high biodegradation rate of MC-RR. With the cell-free extract (CE) of Sphingopyxis sp. USTB-05, MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain, so that initial MC-RR of 42.3 mg&;#8226;L－1 was completely biodegraded within 10 h. During the bio-reaction of MC-RR catalyzed by CE, two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm, indicating that the group of Adda in all products of MC-RR remained intact.
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| 关 键 词： | face=Verdana>microcystin-RR Sphingopyxis sp. USTB-05 cell-free extract biodegradation |
| 收稿时间： | 2009-2-10 |
| 修稿时间： | 2009-2-10
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Biodegradation of microcystin-RR by a new isolated Sphingopyxis sp. USTB-05 |
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| WANG Junfeng,WU Pengfei,CHEN Jian,YAN Hai.Biodegradation of microcystin-RR by a new isolated Sphingopyxis sp. USTB-05[J].Chinese Journal of Chemical Engineering,2010,18(1):108-112. |
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| Authors: | WANG Junfeng WU Pengfei CHEN Jian YAN Hai |
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| Affiliation: | Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Bei-jing 100083, China Department of Environmental Engineering, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, China |
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| Abstract: | A promising bacterial strain for the biodegradation of Microcystins (MCs) was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3 mg·L~(-1) was completely degraded by USTB-05 within 36 h, which was a relatively high biodegradation rate of MC-RR.With the cell-free extract (CE) of Sphingopyxis sp.USTB-05, MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain, so that initial MC-RR of 42.3 mg·L~(-1) was completely biodegraded within 10 h.During the bio-reaction of MC-RR catalyzed by CE, two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm, indicating that the group of Adda in all products of MC-RR remained intact. |
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| Keywords: | microcystin-RR Sphingopyxis sp USTB-05 cell-free extract biodegradation |
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